?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F8328%2F&rft.title=Point-Of-Care+Sensors+for+Diabetes+and+Myocardial+Ischemia&rft.creator=Baca%2C+Justin+Thomas&rft.description=Cardiovascular+disease+is+the+leading+cause+of+death+in+the+United+States%2C+and+over+20+million+people+in+this+country+currently+have+diabetes.+We+have+developed+new+analytical+methods+that+have+the+potential+to+improve+the+monitoring+and+detection+of+these+diseases.+Our+work+enables+point-of-care+sensing+for+biochemical+markers+of+these+diseases%2C+advancing+the+non-invasive+monitoring+of+diabetes+and+the+rapid+detection+of+myocardial+ischemia.+Close+monitoring+of+blood+glucose+concentration+is+critical+in+the+management+of+diabetes.+A+recent+approach+to+non-invasive+monitoring+is+to+measure+glucose+concentration+in+tear+fluid.+However%2C+the+relationship+between+tear+and+blood+glucose+concentration+is+poorly+understood.+We+have+developed+an+analytical+method+for+determining+tear+glucose+concentration%2C+and+have+used+it+to+help+better+define+the+correlation+between+tear+and+blood+glucose+in+both+fasting+and+non-fasting+human+subjects.+We+also+reviewed+the+tear+glucose+literature+from+the+last+80+years%2C+and+have+used+our+method+to+help+explain+some+of+the+discrepancies+reported.+This+work+will+aid+in+developing+contact+lens-based+sensors+for+tear+glucose%2C+and+may+help+to+decrease+or+eliminate+finger-stick+testing.The+definitive+detection+of+myocardial+ischemia+is+difficult.+While+some+patients+are+sent+home+with+an+undetected+heart+attack%2C+many+are+unnecessarily+admitted+to+the+hospital%2C+costing+the+medical+system+~%2412+billion+a+year.+The+Cobalt+Binding+Assay+is+a+test+that+has+shown+promise+in+ruling+out+myocardial+ischemia.+However+it+is+limited+by+the+need+to+access+a+central+clinical+chemistry+laboratory.+We+have+developed+a+Polymerized+Crystalline+Colloidal+Array+(PCCA)+sensor+that+could+be+used+at+the+bedside+to+rapidly+detect+myocardial+ischemia.+This+sensor+uses+photonic+crystal+and+hydrogel+technology+to+actuate+a+color+change+in+response+to+metal+cation+binding+affinity+changes+indicative+of+ischemia.+Specifically%2C+the+sensors+monitor+a+decrease+in+the+binding+affinity+of+human+plasma+for+metals+such+as+Ni2%2B+and+Co2%2B%2C+which+has+been+shown+to+correlate+with+recent+ischemia.+We+have+also+developed+improved+methods+for+fabricating+and+testing+the+PCCA+sensors+that+are+applicable+to+implementing+point-of-care+sensors+for+a+wide+variety+of+analytes.&rft.date=2007-09-19&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F8328%2F1%2FBacaJT_ETD2007pitt.pdf&rft.identifier=++Baca%2C+Justin+Thomas++(2007)+Point-Of-Care+Sensors+for+Diabetes+and+Myocardial+Ischemia.++Doctoral+Dissertation%2C+University+of+Pittsburgh.++++(Unpublished)++