eprintid: 8216
rev_number: 4
userid: 6
dir: disk0/00/00/82/16
datestamp: 2011-11-10 19:48:58
lastmod: 2016-11-15 13:45:11
status_changed: 2011-11-10 19:48:58
type: thesis_degree
metadata_visibility: show
contact_email: wish43043@gmail.com
item_issues_count: 0
eprint_status: archive
creators_name: CHO,  HYO SOON
creators_email: wish43043@gmail.com
title: Diffusion and Solid State NMR Studies of Structures in Model Biological Membranes
ispublished: unpub
divisions: sch_as_chemistry
full_text_status: public
keywords: bicelle; diffusion
abstract: We studied the structures in model biological membranes using solid state NMR and NMR diffusion microscopy. We have created a stable bicelle system containing cholesterol, unsaturated and saturated lipids, capable of forming micron-scale lipid domains and suitable for structural biology studies of membrane proteins. The domains were observed by measuring time-dependent diffusion constants reflecting restricted diffusion of the lipids within micron-scale regions of the bicelles. We observed the correlation of bilayer structure of diverse bicelle systems with their alignment capability, using 31P NMR to measure the density of perforations in the bilayer as a function of bicelle alignment. We found a critical density for optimal bicelle alignment, applicable to bicelles of different lipid compositions. We were able to measure the effect of the perforation density on micron-scale domain formation in lipid bilayers. The perforations redistributed line active lipids within the bilayer, affecting the bulk line tension of the lipid domain. This work demonstrated that membrane topology can control domain formation.
date: 2011-09-16
date_type: completed
institution: University of Pittsburgh
refereed: TRUE
etdcommittee_type: committee_chair
etdcommittee_type: committee_member
etdcommittee_type: committee_member
etdcommittee_type: committee_member
etdcommittee_name: Spence, Megan M.
etdcommittee_name: Waldeck, David
etdcommittee_name: van der Wel, Patrick
etdcommittee_name: Saxena, Sunil
etdcommittee_email: mmspence@gmail.com
etd_defense_date: 2011-06-23
etd_approval_date: 2011-09-16
etd_submission_date: 2011-06-28
etd_access_restriction: immediate
etd_patent_pending: FALSE
assigned_doi: doi:10.5195/pitt.etd.2011.8216
thesis_type: dissertation
degree: PhD
committee: Megan M. Spence (mmspence@gmail.com) - Committee Chair
committee: David Waldeck () - Committee Member
committee: Patrick van der Wel () - Committee Member
committee: Sunil Saxena () - Committee Member
etdurn: etd-06282011-163133
other_id: http://etd.library.pitt.edu/ETD/available/etd-06282011-163133/
other_id: etd-06282011-163133
citation:   CHO, HYO SOON  (2011) Diffusion and Solid State NMR Studies of Structures in Model Biological Membranes.  Doctoral Dissertation, University of Pittsburgh.    (Unpublished)  
document_url: http://d-scholarship-dev.library.pitt.edu/8216/1/06232011Cho.pdf