TY  - UNPB
ID  - pittir8216
UR  - http://d-scholarship-dev.library.pitt.edu/8216/
A1  - CHO,  HYO SOON
TI  - Diffusion and Solid State NMR Studies of Structures in Model Biological Membranes
Y1  - 2011/09/16/
N2  - We studied the structures in model biological membranes using solid state NMR and NMR diffusion microscopy. We have created a stable bicelle system containing cholesterol, unsaturated and saturated lipids, capable of forming micron-scale lipid domains and suitable for structural biology studies of membrane proteins. The domains were observed by measuring time-dependent diffusion constants reflecting restricted diffusion of the lipids within micron-scale regions of the bicelles. We observed the correlation of bilayer structure of diverse bicelle systems with their alignment capability, using 31P NMR to measure the density of perforations in the bilayer as a function of bicelle alignment. We found a critical density for optimal bicelle alignment, applicable to bicelles of different lipid compositions. We were able to measure the effect of the perforation density on micron-scale domain formation in lipid bilayers. The perforations redistributed line active lipids within the bilayer, affecting the bulk line tension of the lipid domain. This work demonstrated that membrane topology can control domain formation.
AV  - public
KW  - bicelle; diffusion
ER  -