?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F7993%2F&rft.title=SINGLE-CELL+ELECTROPORATION+USING+ELECTROLYTE-FILLED+CAPILLARIES+WITH+MICRO-SCALE+TIPS&rft.creator=Wang%2C+Manyan&rft.description=Single-cell+electroporation+(SCEP)+is+a+recently+developing+powerful+technique+for+cell+analysis+and+cell+manipulation.+In+the+first+chapter+of+this+thesis%2C+a+review+about+the+theories+and+techniques+is+fulfilled%2C+including+a+detailed+description+of+the+factors+affecting+SCEP%2C+and+a+discussion+about+how+to+optimize+SCEP+for+high+efficiency+and+survivability.+Based+on+the+previous+experimental+results+and+numerical+simulation%2C+a+hypothesis+is+proposed+which+leads+us+to+find+that+small+tips+could+be+a+solution+to+electroporate+small+cells+with+simultaneous+maximization+of+electroporation+efficiency+and+survivability+when+using+electrolyte-filled+capillaries+(EFC)+with+pulled+tips.In+the+second+chapter%2C+an+integrated+circuit+for+SCEP+and+controlling+is+demonstrated.+EFCs+with+2+%26mu+tips+are+constructed+and+used+for+SCEP+of+A549+cells+with+an+extremely+high+spatial+resolution.+Distance+between+tip+and+cell+is+revealed+to+be+vital+in+SCEP+because+of+its+direct+control+of+the+local+electric+field+distribution+and+strength%3B+to+control+distance+precisely%2C+a+current+measurement+method+inspired+by+tip-cell+giga-seals+is+applied.+High+temporal+resolution+videos+hint+an+abrupt+intracellular+fluorescence+loss+at+the+time+scale+of+pulse+duration+followed+by+recovery+in+the+small+portion+of+cell+membrane+facing+the+tips.+Viability+of+cells+is+highly+related+to+the+fluorescence+loss%2C+fluorescence+exposure+and+dye+types.+Comsol+simulation+using+the+real+shape+capillaries+helps+to+guide+the+electroporation+throughout+our+experiments.However%2C+this+protocol+evokes+overcoming+technical+difficulties+in+terms+of+getting+high+survivability+and+decreasing+variance%2C+which+are+our+two+main+aims.+The+advantage+of+small+tips+and+the+hypothesis+are+still+to+be+examined.+This+is+referred+in+the+third+chapter%2C+as+well+as+other+following-up+future+work.&rft.date=2008-09-28&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F7993%2F1%2FWangManyan23June2008.pdf&rft.identifier=++Wang%2C+Manyan++(2008)+SINGLE-CELL+ELECTROPORATION+USING+ELECTROLYTE-FILLED+CAPILLARIES+WITH+MICRO-SCALE+TIPS.++Master's+Thesis%2C+University+of+Pittsburgh.++++(Unpublished)++