eprintid: 7684
rev_number: 4
userid: 6
dir: disk0/00/00/76/84
datestamp: 2011-11-10 19:42:49
lastmod: 2016-11-15 13:42:45
status_changed: 2011-11-10 19:42:49
type: thesis_degree
metadata_visibility: show
contact_email: lew16@pitt.edu
item_issues_count: 0
eprint_status: archive
creators_name: Wang, Lei
creators_email: lew16@pitt.edu
creators_id: LEW16
title: The Denaturation of Cytochrome c and Cytochrome c as Peroxidase
ispublished: unpub
divisions: sch_as_chemistry
full_text_status: public
keywords: cyclic voltammogram; cytochrome c; gold ball electode; hydrogen peroxide
abstract: Conformational transitions of proteins play a crucial role in many biochemical and biophysical reactions. Understanding the conformational changes of a protein upon adsorption to a substrate is very important in biotechnology, e.g. the development of modern protein chip technology, biocompatibility of implants, and many other ones. This research program used cytochrome c, an electron carrier in the respiratory chain, as a model to probe how surface adsorption affects the folding of a protein. My work investigates the interaction of protein horse heart cytochrome c when it is electrostatically adsorbed or covalently attached onto a 1 nm thick monolayer film, which covers an Au surface. After changing the pH value of the solutions or adding the denaturants into the solutions, the conformation of cytochrome c changes and causes a change of the peak current of cyclic voltammgram. In addition, the denatured cytochrome c¡¯s peroxidase activity will be studied and compared with the peroxidase activity of microperoxidase-11.
date: 2006-09-28
date_type: completed
institution: University of Pittsburgh
refereed: TRUE
etdcommittee_type: committee_chair
etdcommittee_type: committee_member
etdcommittee_type: committee_member
etdcommittee_name: Waldeck, David H
etdcommittee_name: Michael, Adrian C
etdcommittee_name: Saxena, Sunil K
etdcommittee_email: dave@pitt.edu
etdcommittee_email: amichael@pitt.edu
etdcommittee_email: sksaxena@pitt.edu
etdcommittee_id: DAVE
etdcommittee_id: AMICHAEL
etdcommittee_id: SKSAXENA
etd_defense_date: 2006-01-26
etd_approval_date: 2006-09-28
etd_submission_date: 2006-04-27
etd_access_restriction: immediate
etd_patent_pending: FALSE
assigned_doi: doi:10.5195/pitt.etd.2011.7684
thesis_type: thesis
degree: MS
committee: David H. Waldeck (dave@pitt.edu) - Committee Chair
committee: Adrian C. Michael (amichael@pitt.edu) - Committee Member
committee: Sunil K. Saxena (sksaxena@pitt.edu) - Committee Member
etdurn: etd-04272006-170820
other_id: http://etd.library.pitt.edu/ETD/available/etd-04272006-170820/
other_id: etd-04272006-170820
citation:   Wang, Lei  (2006) The Denaturation of Cytochrome c and Cytochrome c as Peroxidase.  Master's Thesis, University of Pittsburgh.    (Unpublished)  
document_url: http://d-scholarship-dev.library.pitt.edu/7684/1/WangL_etdPitt_2006.pdf