?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F7420%2F&rft.title=UV+RAMAN+INVESTIGATION+OF+PEPTIDEHYDRATION+AND+HYDROPHOBIC+COLLAPSE&rft.creator=Pimenov%2C+Konstantin+Valentinovich&rft.description=Hydration+of+an+alpha-helical%2C+21+residue%2C+mainly+Ala+peptide+was+investigated+by+UV+resonance+Raman+(UVR)+spectroscopy.+UVR+spectra+in+the+dehydrated+solid+state+were+compared+to+those+in+aqueous+solution+at+different+temperatures.+The+amide+band+frequencies+of+a+dehydrated+solid+alpha-helix+peptide+show+frequency+shifts+compared+to+those+in+aqueous+solution+due+to+the+loss+of+amide+backbone+hydrogen+bonding+to+water%3B+the+amide+II+and+amide+III+bands+of+the+solid+alpha-helix+downshift%2C+while+the+amide+I+band+upshifts.+The+shifts+are+identical+in+direction+but+smaller+than+those+that+occur+for+alpha-helices+in+aqueous+solution+as+the+temperature+increases%3B+water+hydrogen+bonding+strengths+decrease+as+the+temperature+increases.+The+UV+Raman+amide+band+frequency+shifts+can+be+used+to+monitor+alpha-helix+hydrogen+bonding.In+addition%2C+the+phenomenon+of+hydrophobic+collapse+was+investigated+in+poly(N-isopropylacrylamide)+hydrogel+nanoparticles.+~100+nm+hydrogel+particles+were+synthesized+and+analyzed+with+variety+of+methods+including+UVR+spectroscopy.+The+changes+in+UVR+amide+spectra+are+similar+to+those+observed+for+peptide+dehydration.+The+Raman+bands+associated+with+C-H+stretching+vibrations+change+in+a+manner+expected+to+dehydration+as+well.+Comparison+of+spectral+changes+of+isopropyl+group+vibrations+to+that+of+amide+group+suggests+different+dehydration+pattern+of+the+two+groups.+Kinetics+of+the+dehydration+of+the+amide+groups+indicates+a+complex+process%2C+which+can+not+be+described+by+a+two+state+model.&rft.date=2007-06-14&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F7420%2F1%2FPimenov_KV_Apr_2007.pdf&rft.identifier=++Pimenov%2C+Konstantin+Valentinovich++(2007)+UV+RAMAN+INVESTIGATION+OF+PEPTIDEHYDRATION+AND+HYDROPHOBIC+COLLAPSE.++Master's+Thesis%2C+University+of+Pittsburgh.++++(Unpublished)++