eprintid: 7338
rev_number: 4
userid: 6
dir: disk0/00/00/73/38
datestamp: 2011-11-10 19:39:14
lastmod: 2016-11-15 13:41:06
status_changed: 2011-11-10 19:39:14
type: thesis_degree
metadata_visibility: show
contact_email: stopper@ppg.com
item_issues_count: 0
eprint_status: archive
creators_name: Topper, Sarah L.
creators_email: stopper@ppg.com
title: Extra-column Band Broadening in Ultra High Performance Liquid Chromatography
ispublished: unpub
divisions: sch_as_chemistry
full_text_status: public
keywords: capillary; Gaussian; injector; variance
abstract: Advances in column technologies for high performance liquid chromatography (HPLC) have led to the use of small, highly efficient packing materials. The use of these materials requires short, small diameter columns as well as instruments capable of withstanding high pressures (up to 1000bar) and sometimes temperature (in excess of 100°C), a technique dubbed ultra-high performance liquid chromatography or UPLC. The advantage is a greater than ten-fold reduction in analysis time without a loss of peak capacity or resolution. Due to the small volumes inherent in the new columns, the extra-column volumes of the instrument can become a significant source of dispersion leading to extra-column broadening of chromatographic peaks. Uncontrolled or accounted for, this variance severely limits the separation potential of improved column packings and reduces the accuracy of evaluations of instruments and columns. An investigation is made of the source and nature of the band broadening in instrumental components with an eye towards reduction of variance without loss of performance. Different methods for calculating the degree of extra-column band broadening are discussed. Applications of the calculated data for evaluation of the kinetic parameters of UPLC are reviewed.
date: 2011-06-06
date_type: completed
institution: University of Pittsburgh
refereed: TRUE
etdcommittee_type: committee_chair
etdcommittee_type: committee_member
etdcommittee_type: committee_member
etdcommittee_name: Weber, Stephen G
etdcommittee_name: Michael, Adrian C
etdcommittee_name: Trakselis, Michael A
etdcommittee_email: sweber@pitt.edu
etdcommittee_email: amichael@pitt.edu
etdcommittee_email: mtraksel@pitt.edu
etdcommittee_id: SWEBER
etdcommittee_id: AMICHAEL
etdcommittee_id: MTRAKSEL
etd_defense_date: 2011-04-19
etd_approval_date: 2011-06-06
etd_submission_date: 2011-04-19
etd_access_restriction: immediate
etd_patent_pending: FALSE
assigned_doi: doi:10.5195/pitt.etd.2011.7338
thesis_type: thesis
degree: MS
committee: Stephen G. Weber (sweber@pitt.edu) - Committee Chair
committee: Adrian C. Michael (amichael@pitt.edu) - Committee Member
committee: Michael A. Trakselis (mtraksel@pitt.edu) - Committee Member
etdurn: etd-04192011-120702
other_id: http://etd.library.pitt.edu/ETD/available/etd-04192011-120702/
other_id: etd-04192011-120702
citation:   Topper, Sarah L.  (2011) Extra-column Band Broadening in Ultra High Performance Liquid Chromatography.  Master's Thesis, University of Pittsburgh.    (Unpublished)  
document_url: http://d-scholarship-dev.library.pitt.edu/7338/1/toppersl_etd2011.pdf