eprintid: 40086
rev_number: 12
userid: 10137
dir: disk0/00/04/00/86
datestamp: 2021-01-20 18:33:29
lastmod: 2021-01-20 18:33:29
status_changed: 2021-01-20 18:33:29
type: thesis_degree
succeeds: 39949
metadata_visibility: show
contact_email: duvallsam1@gmail.com
item_issues_id: thesis_degree_versioning
item_issues_id: duplicate_title_39868
item_issues_id: duplicate_title_39948
item_issues_id: duplicate_title_39949
item_issues_type: thesis_degree_versioning
item_issues_type: duplicate_title
item_issues_type: duplicate_title
item_issues_type: duplicate_title
item_issues_description: ETD 40086 is using versioning.
item_issues_description: Duplicate title to
Duvall, Samuel
Development of methods to study bacterial cell signaling in vivo.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
item_issues_description: Duplicate title to
Duvall, Samuel
Development of methods to study bacterial cell signaling in vivo.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
item_issues_description: Duplicate title to
Duvall, Samuel
Development of methods to study bacterial cell signaling in vivo.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
item_issues_timestamp: 2020-11-11 07:02:23
item_issues_timestamp: 2020-11-30 07:02:25
item_issues_timestamp: 2020-11-30 07:02:25
item_issues_timestamp: 2020-12-13 07:02:25
item_issues_status: discovered
item_issues_status: autoresolved
item_issues_status: autoresolved
item_issues_status: autoresolved
item_issues_count: 1
eprint_status: archive
creators_name: Duvall, Samuel
creators_email: swd11@pitt.edu
creators_id: swd11
title: Development of methods to study bacterial cell signaling in vivo
ispublished: unpub
divisions: sch_as_chemistry
full_text_status: restricted
keywords: N/A
abstract: Our understanding of bacterial signaling systems is still in its early stages. As we learn more about how bacteria respond to environmental and intracellular cues, we will be able to tackle some of the most important problems related to bacteria. Drug-resistance, agriculture, environmental remediation, and more global problems will be better understood and potentially solved in some way by our understanding of bacterial signaling systems. Two-component systems are the foundation of bacterial signaling. Currently, we lack tools for studying two-component signaling systems in vivo and with greater depth than simply whether two proteins colocalize or not. In this dissertation, I will explore the development of tools to study members of two-component signaling systems and the proteins that scaffold them.
Chapter 2 will explore the first example of a histidine kinase FRET sensor which showcases the potential for this type of FRET sensor for any histidine kinase. I provide details on its design and controls for working with it on conventional epifluorescent microscopes. Chapter 3 expands on the use of the CckA-FRET biosensor and previously reported leucine zipper technology to understand the domain interactions of the pseudokinase DivL with the histidine kinase CckA. In Chapter 4, I apply knowledge of histidine kinases to the current antibiotic crisis.
Scaffolding proteins play a multitude of roles in bacteria. In Chapter 5 I will explore PodJ, the multirole scaffolding protein that can recruit PopZ in order to add depth to a signaling network. Chapter 6 will further look at an interesting phenotype of PodJ and how it relates to self-assembly and curvature recognition. Chapter 6 will also cover new tool development for working in C. crescentus.
In each chapter, I will cover questions and future aims. This dissertation overall is aimed at the development of new ways of thinking about bacterial signaling systems and how to study them.
date: 2021-01-20
date_type: published
pages: 349
institution: University of Pittsburgh
refereed: TRUE
etdcommittee_type: committee_chair
etdcommittee_type: committee_member
etdcommittee_type: committee_member
etdcommittee_type: committee_member
etdcommittee_name: Childers, Seth
etdcommittee_name: Saxena, Sunil
etdcommittee_name: Weber, Steve
etdcommittee_name: McCormick, Joseph
etdcommittee_email: wschild@pitt.edu
etdcommittee_email: sksaxena@pitt.edu
etdcommittee_email: sweber@pitt.edu
etdcommittee_email: mccormick@duq.edu
etdcommittee_id: wschild
etdcommittee_id: sksaxena
etdcommittee_id: sweber
etd_defense_date: 2020-11-23
etd_approval_date: 2021-01-20
etd_submission_date: 2020-10-26
etd_release_date: 2021-01-20
etd_access_restriction: 2_year
etd_patent_pending: FALSE
thesis_type: dissertation
degree: PhD
citation: Duvall, Samuel (2021) Development of methods to study bacterial cell signaling in vivo. Doctoral Dissertation, University of Pittsburgh. (Unpublished)
document_url: http://d-scholarship-dev.library.pitt.edu/40086/45/Samuel%20Duvall%20Final%20ETD.pdf