?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F40086%2F&rft.title=Development+of+methods+to+study+bacterial+cell+signaling+in+vivo&rft.creator=Duvall%2C+Samuel&rft.description=Our+understanding+of+bacterial+signaling+systems+is+still+in+its+early+stages.+As+we+learn+more+about+how+bacteria+respond+to+environmental+and+intracellular+cues%2C+we+will+be+able+to+tackle+some+of+the+most+important+problems+related+to+bacteria.+Drug-resistance%2C+agriculture%2C+environmental+remediation%2C+and+more+global+problems+will+be+better+understood+and+potentially+solved+in+some+way+by+our+understanding+of+bacterial+signaling+systems.+Two-component+systems+are+the+foundation+of+bacterial+signaling.+Currently%2C+we+lack+tools+for+studying+two-component+signaling+systems+in+vivo+and+with+greater+depth+than+simply+whether+two+proteins+colocalize+or+not.+In+this+dissertation%2C+I+will+explore+the+development+of+tools+to+study+members+of+two-component+signaling+systems+and+the+proteins+that+scaffold+them.+%0D%0AChapter+2+will+explore+the+first+example+of+a+histidine+kinase+FRET+sensor+which+showcases+the+potential+for+this+type+of+FRET+sensor+for+any+histidine+kinase.+I+provide+details+on+its+design+and+controls+for+working+with+it+on+conventional+epifluorescent+microscopes.+Chapter+3+expands+on+the+use+of+the+CckA-FRET+biosensor+and+previously+reported+leucine+zipper+technology+to+understand+the+domain+interactions+of+the+pseudokinase+DivL+with+the+histidine+kinase+CckA.+In+Chapter+4%2C+I+apply+knowledge+of+histidine+kinases+to+the+current+antibiotic+crisis.%0D%0A%09Scaffolding+proteins+play+a+multitude+of+roles+in+bacteria.+In+Chapter+5+I+will+explore+PodJ%2C+the+multirole+scaffolding+protein+that+can+recruit+PopZ+in+order+to+add+depth+to+a+signaling+network.+Chapter+6+will+further+look+at+an+interesting+phenotype+of+PodJ+and+how+it+relates+to+self-assembly+and+curvature+recognition.+Chapter+6+will+also+cover+new+tool+development+for+working+in+C.+crescentus.%0D%0A%09In+each+chapter%2C++I+will+cover+questions+and+future+aims.+This+dissertation+overall+is+aimed+at+the+development+of+new+ways+of+thinking+about+bacterial+signaling+systems+and+how+to+study+them.&rft.date=2021-01-20&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F40086%2F45%2FSamuel%2520Duvall%2520Final%2520ETD.pdf&rft.identifier=++Duvall%2C+Samuel++(2021)+Development+of+methods+to+study+bacterial+cell+signaling+in+vivo.++Doctoral+Dissertation%2C+University+of+Pittsburgh.++++(Unpublished)++