?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F39476%2F&rft.title=Theoretical+and+Experimental+Improvements+for+Fast+Microdialysis&rft.creator=Ngo%2C+Khanh&rft.description=Microdialysis+is+the+most+widely+used+technique+for+intracranial+sampling.+Its+versatility+prompts+important+applications%2C+from+bedside+traumatic+brain+injury+monitoring+to+psychological+disorder+treatment.+However%2C+historically+microdialysis+methods+have+a+time+resolution+of+5-30+mins%2C+and+thus+are+not+well+suited+to+obtain+physiological+information%2C+such+as+rates+of+extracellular+processes+or+the+relationship+between+neurochemical+levels+and+behavior.+Building+from+recent+work+in+our+laboratory%2C+we+developed+instrumentation+for+making+dopamine+measurements+in+awake+and+freely+moving+rats+over+extended+periods+at+one-minute+time+resolution+using+microdialysis+sampling+analyzed+with+online+HPLC+(Fast+Microdialysis).%0D%0AFast+Microdialysis+was+used+to+investigate+the+beneficial+effects+of+the+anti-inflammatory+drug+dexamethasone+(DEX)+to+penetration+injury+caused+by+dialysis+probe+implantation.+Retrodialysis+of+DEX+was+found+to+potentiate+both+basal+levels+and+stimulated+release+of+striatal+DA.+Applying+Fast+Microdialysis+to+behavioral+studies+necessitated+creation+of+a+rotating+operant+chamber.+Using+this+device%2C+both+trained-+and+untrained-+animal%E2%80%99s+behavior+correlated+with+DA+release%2C+however+with+different+characteristics.+Robust+and+fast+determination+of+DA+allowed+for+creation+of+new+microdialysis+techniques+in+the+non-steady+state+regime+to+investigate+morphology+and+neurotransmitter+regulation.+Thus%2C+a+comprehensive+mathematical+model+was+created+to+analyze+transient+responses+measured+by+Fast+Microdialysis.+A+robust%2C+adaptive+random+sampling+simplex+approach+was+used+to+fit+the+model+to+transient+data.+Striatal+tissue+tortuosity%2C+porosity+and+the+reuptake+rate+constant+of+DA+were+determined+from+a+single+transient+response+on+awake+and+freely+moving+rats.&rft.date=2020-09-16&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=video%2Fmp4&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F39476%2F14%2FVideo2.mp4&rft.format=video%2Fmp4&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F39476%2F15%2FVideo1.mp4&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F39476%2F22%2FKhanh%2520Ngo%2520Final%2520ETD.pdf&rft.identifier=++Ngo%2C+Khanh++(2020)+Theoretical+and+Experimental+Improvements+for+Fast+Microdialysis.++Doctoral+Dissertation%2C+University+of+Pittsburgh.++++(Unpublished)++