?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F39024%2F&rft.title=Relationship+Between+Single+Nucleotide+Polymorphisms+and+Severe+Dengue+in+a+Brazilian+Population&rft.creator=Kamasa-Quashie%2C+Dzigbordi&rft.description=Dengue+virus+has+become+one+of+the+most+important+arboviral+diseases+of+today.+With+nearly+half+of+the+global+population+at+risk%2C+this+infectious+disease+carries+great+significance.+The+aim+of+this+study+was+to+determine+the+relationship+between+18+single+nucleotide+polymorphisms+(SNPs)+and+severe+dengue+in+a+population+from+Recife%2C+Brazil.+The+SNPs+of+interest+are+as+follows%3A+TLR8+rs17256081%2C+IFNG+rs2069718%2C+IFNG+rs2069727%2C+IRF1+rs2070729%2C+OAS2+rs2072137%2C+OAS2+rs2072138%2C+OAS3+rs2240188%2C+MX1+rs3737399%2C+VEPH1+rs3911403%2C+IRAK4+rs4251580%2C+CLEC4C+rs17199006%2C+PLCE1+rs3740360%2C+MRC1+rs606231248%2C+MRC1+rs2296414%2C+RNASEL+rs486907%2C+OASL+rs3213545%2C+MX1+rs7277299%2C+and+MICB+rs3132468.+A+total+of+450+DNA+samples+were+pulled+from+two+studies%E2%80%94a+cohort+study+of+dengue+patients+and+a+yellow+fever+vaccine+cohort.+Sample+concentrations+were+tested+using+the+Nanodrop+1000+Spectrometer.+The+concentrations+of+all+samples+were+between+10-100+ng%2FuL%2C+per+the+laboratory+technician%E2%80%99s+request.+Samples+were+transported+to+the+University+of+Pittsburgh%E2%80%99s+Genomic+Core+Research+Laboratory+for+genotyping+using+the+iPlex+MassARRAY+system+and+results+were+analyzed+using+Microsoft+Excel+and+R+statistical+software.+Of+the+18+SNPs%2C+statistically+significant+results+were+observed+for+OAS2+rs2072137%2C+OAS3+rs2240188%2C+PLCE1+rs3740360%2C+and+MX1+rs7277299.+For+OAS2+rs2072137%2C+the+CC+genotype+was+shown+to+be+significantly+associated+with+severe+dengue+(OR%3D2.10%2C+P%3D0.01).+The+CC+genotype+associated+with+OAS3+rs2240188+also+appears+to+influence+disease+severity+(OR%3D1.96%2C+P%3D0.02).+For+PLCE1+rs3740360%2C+calculations+reveal+a+significant+association+between+the+AA+genotype+and+severe+dengue+(OR%3D2.28%2C+P%3D0.03).+The+last+notable+result+was+found+in+MX1+rs7277299+(OR%3D5.33%2C+P%3D0.02)+where+the+CC+genotype+was+also+significantly+associated+with+severe+disease.+Though+this+is+one+of+the+largest+dengue-related+gene+association+studies%2C+further+research+is+necessary+to+validate+the+findings.%09+The+increasing+burden+of+dengue+disease+signifies+the+public+health+importance+of+this+research%E2%80%94to+contribute+to+the+advancement+of+dengue+research%2C+vaccine+development%2C+therapeutic+strategies%2C+and+diagnostic+tools.&rft.date=2020-07-30&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F39024%2F1%2FKamasaQuashie_D_MPHthesis_4_2020.pdf&rft.identifier=++Kamasa-Quashie%2C+Dzigbordi++(2020)+Relationship+Between+Single+Nucleotide+Polymorphisms+and+Severe+Dengue+in+a+Brazilian+Population.++Master's+Thesis%2C+University+of+Pittsburgh.++++(Unpublished)++