<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Tissue specific Cish expression supports alveolar macrophage homeostatic function"^^ . "Macrophages play critical roles in defense against microbes and clearance of dead cells, but also perform tissue specific functions in homeostasis. Distinct gene expression signatures in macrophages isolated from varying tissues are largely determined by environmental signals. Specifically, the lung is highly susceptible to environmental changes, such as O2 pressure and inhalation of particulate and microbes. Alveolar macrophages are shaped by the lung environment and have critical tissue-specific functions in initiating and resolving lung inflammation, and in maintaining lung structure via surfactant and lipid catabolism. While research speculates lung specific factors form alveolar macrophage phenotype and homeostatic function, the specific signals and regulators remain largely unknown. Therefore, we sought to explore lung specific cytokine signals and downstream signaling regulators that shape homeostatic functions of alveolar macrophages.\r\nWe found Cytokine Inducible SH2 Containing Protein (Cish), a SOCS family member known to regulate the JAK-STAT5 pathway, is basally expressed in a tissue-specific manner in alveolar macrophages. Further, we found that the STAT5 activating cytokine GM-CSF regulates Cish expression in alveolar macrophages and observed reduced alveolar macrophage Cish expression with GM-CSF blockade in the lung. Cish knockout mice exhibit enlarged “foamy” alveolar macrophages, impaired surfactant metabolism, and dysregulated response to GM-CSF, all hallmarks of pulmonary alveolar proteinosis. Thus, we show alveolar macrophage Cish expression is directly linked to lung specific factors, namely GM-CSF, and influences surfactant homeostasis in the lung, a critical homeostatic function of alveolar macrophages. \r\nThe lung is an especially critical site of protection as it is a barrier site that is constantly exposed to inhaled particulate and microbes and possesses a fragile structure. Alveolar macrophages act as sentinels in the lung, protecting this sensitive tissue from challenge while maintaining proper homeostasis and structure. From a public health perspective, continuing to elucidate the specific mechanisms by which alveolar macrophages mediate lung homeostasis is essential to providing cutting edge health care and to continuing to develop therapeutic treatments that can provide cures instead of simply mitigating symptoms of pulmonary disease. Here, we highlight one of many yet to be uncovered regulators of lung homeostasis."^^ . "2020-01-29" . . . . . . . . "Karsen E."^^ . "Shoger"^^ . "Karsen E. Shoger"^^ . . . . . . "Tissue specific Cish expression supports alveolar macrophage homeostatic function (PDF)"^^ . . . . . "Shoger_Karsen_MasterThesis_Dec2019.pdf"^^ . . . "Tissue specific Cish expression supports alveolar macrophage homeostatic function (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Tissue specific Cish expression supports alveolar macrophage homeostatic function (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Tissue specific Cish expression supports alveolar macrophage homeostatic function (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Tissue specific Cish expression supports alveolar macrophage homeostatic function (Other)"^^ . . . . . . "small.jpg"^^ . . . "Tissue specific Cish expression supports alveolar macrophage homeostatic function (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #37796 \n\nTissue specific Cish expression supports alveolar macrophage homeostatic function\n\n" . "text/html" . .