eprintid: 34996
rev_number: 16
userid: 7544
dir: disk0/00/03/49/96
datestamp: 2018-09-20 14:36:56
lastmod: 2018-09-20 14:36:56
status_changed: 2018-09-20 14:36:56
type: thesis
metadata_visibility: show
contact_email: j.feldmiller@gmail.com
eprint_status: archive
creators_name: Feldmiller, Joshua
creators_email: jmf168@pitt.edu
creators_id: jmf168
contributors_type: committee_chair
contributors_type: committee_member
contributors_name: Mattila, Joshua
contributors_name: Deslouches, Berthony
contributors_email: jmattila@pitt.edu
contributors_email: tdesl19@pitt.edu
contributors_id: jmattila
contributors_id: tdesl19
title: Assessing the cytotoxicity of select antimicrobial peptides in the treatment of tuberculosis
ispublished: unpub
divisions: sch_gsph_infectiousdiseasesmicrobiology
full_text_status: public
abstract: Tuberculosis (TB) remains a leading cause of death despite the availability of drug treatments.  Antibiotic resistance and genetic mutation has led to the development of both multi drug-resistant and extensively drug-resistant tuberculosis, further complicating successful intervention.  Antimicrobial peptides (AMPs) present a novel alternative to standard antibiotic therapies.  In this study, we used flow cytometry to measure the cytotoxicity of three different AMPs (A4S7, D8 and WLBU2) on three types of host cells associated with Tb infection.  Both WLBU2 and A4S7 were associated with significant cell death (p < 0.05) at and above concentrations of 10μM, and the results for D8 fluctuated by cell type.  The data obtained in this study will inform future replications in bacterial models, from which the plausibility of specific AMPs as treatments for Mycobacterium tuberculosis can be assessed and provide insights of clear public health significance.
date: 2018
date_type: submitted
pages: 35
institution: University of Pittsburgh
refereed: TRUE
thesis_type: masteressay
degree: MPH
citation:   Feldmiller, Joshua  (2018) Assessing the cytotoxicity of select antimicrobial peptides in the treatment of tuberculosis.  Master Essay, University of Pittsburgh.   
document_url: http://d-scholarship-dev.library.pitt.edu/34996/1/Feldmiller_Joshua_M_Aug2018.pdf