?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F31611%2F&rft.title=Analysis+of+peripheral+immune+responses+for+the+development+of+an+encephalitis+non-human+primate+animal+model+for+new+world+alphaviruses&rft.creator=Salama%2C+Noah&rft.description=The+New+World+alphaviruses+eastern+equine+encephalitis+virus+(EEEV)%2C+Venezuelan+equine+encephalitis+virus+(VEEV)%2C+and+western+equine+encephalitis+virus+(WEEV)+are+all+mosquito-borne+pathogens+originating+in+North+and+South+America.+All+three+viruses+are+capable+of+causing+severe+illness+in+animals+and+humans%2C+with+extreme+cases+leading+to+encephalitis.+Given+the+lack+of+commercially+available+vaccines+or+treatments%2C+and+previous+research+into+these+pathogens+as+potential+bioterrorism+weapons%2C+all+three+are+of+great+significance+to+public+health.+The+aim+of+our+laboratory+was+to+develop+a+cynomolgus+macaque+model+to+study+the+unique+neuropathology+of+each+virus+when+delivered+via+an+aerosol+route%2C+the+most+likely+delivery+route+for+a+biological+attack.+To+assess+pathogenesis%2C+peripheral+blood+mononuclear+cells+(PBMCs)+were+isolated+from+whole+blood+and+analyzed+by+flow+cytometry.+Inflammation+in+the+central+nervous+system+(CNS)+was+also+evaluated+using+a+LEGENDplex+bead+based+immunological+array.+Results+indicate+a+substantial+increase+in+the+number+of+CCR2%2B+myeloid+cells+in+circulation%2C+a+receptor+important+for+homing+and+migration+to+infected+tissue.+In+addition%2C+inflammatory+cytokines+MCP-1+(CCL-2)%2C+IP-10%2C+IL-6%2C+and+IL-8+were+substantially+upregulated+in+the+CNS+tissues+and+cerebrospinal+fluid+(CSF)+of+lethally+infected+animals.+Collectively%2C+this+suggests+homing+and+migration+of+peripheral+immune+cells+to+the+CNS%2C+and+identifies+potential+markers+for+future+longitudinal+studies+of+alphavirus+disease+progression+and+severity.&rft.date=2017-06-29&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F31611%2F7%2FRevised%2520Thesis.pdf&rft.identifier=++Salama%2C+Noah++(2017)+Analysis+of+peripheral+immune+responses+for+the+development+of+an+encephalitis+non-human+primate+animal+model+for+new+world+alphaviruses.++Master's+Thesis%2C+University+of+Pittsburgh.++++(Unpublished)++