?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F31573%2F&rft.title=Glucose+transporter+expression+in+lung+granulomas+from+Mycobacterium+tuberculosis-infected+monkeys&rft.creator=Liu%2C+Hong&rft.description=TB+remains+to+be+one+of+the+major+threat+to+public+health+as+it+brings+tremendous+mobility+and+mortality+in+developing+countries.+Nevertheless%2C+with+the+help+from+PET%2FCT+imaging+techniques%2C+it+provides+a+fast+and+sensitive+way+of+diagnosing+active+TB+and+meanwhile%2C+it+proves+to+be+valuable+tool+of+studying+TB+inflammation+and+granuloma.+Our+study+focused+on+glucose+transporter+expression+in+the+granuloma+tissues+from+infected+monkeys+as+we+believed+their+expression+would+be+enhanced+as+a+result+of+Warburg+effect+and+increased+inflammation.+It+can+be+the+one+of+the+major+factors+that+drives+uptake+of+18F-FDG+and+give+rise+to+high+SUV+from+PET%2FCT+results.+However%2C+our+preliminary+data+did+not+fully+support+this+hypothesis.+Although+initial+RT-PCR+data+supported+higher+Glut1%2C+Glut3%2C+Glut8+and+Glut14+expression+from+pooled+granuloma+tissue+RNA%2C+further+quantification+study+from+tissues+with+known+SUV+levels+didn%E2%80%99t+not+show+higher+expression+of+glucose+transporters+in+granuloma+tissues+in+compare+to+normal+tissues+except+Glut3.+Individual+comparison+among+glucose+transporters+in+granuloma+tissues+showed+lower+expression+relative+to+HPRT1+except+Glut3+and+most+glucose+transporter+expression+didn%E2%80%99t+not+show+positive+correlation+with+SUV+levels.+Although+tissue+staining+experiment+did+confirmed+higher+expression+of+Glut1+and+Glut3+in+the+granuloma+tissues%2C+MFI+studies+and+statistical+analysis+didn%E2%80%99t+not+support+expression+of+glucose+transporters+as+a+major+contributor+to+the+higher+SUV+levels.+Therefore%2C+there+is+not+enough+evidence+to+conclude+glucose+transporter+expression+in+the+granuloma+tissues+may+be+the+main+factor+that+drives+FDG+uptake+and+responsible+for+different+SUV+levels+from+PET%2FCT+outcome.&rft.date=2017-06-29&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F31573%2F1%2FLiuH_Glucose%2520transporter_etd_april2017.pdf&rft.identifier=++Liu%2C+Hong++(2017)+Glucose+transporter+expression+in+lung+granulomas+from+Mycobacterium+tuberculosis-infected+monkeys.++Master's+Thesis%2C+University+of+Pittsburgh.++++(Unpublished)++