?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F26569%2F&rft.title=Investigation+of+Reactive+Oxygen+Species+Management+in+Biological+and+Pathological+Processes+by+Fluorescence+Imaging&rft.creator=Yin%2C+Bocheng&rft.description=Neural+damage+caused+by+ischemia%2Fstroke+is+initiated+by+excess+reactive+oxygen+species+(ROS).+Different+cell+types+and+even+same+cell+type%2C+but+in+different+regions+in+hippocampus+are+found+to+have+differential+susceptibility+to+ischemia%2Fstroke.+ROS+levels+have+been+found+to+be+different+in+different+cell+types+or+neurons+in+different+regions+of+hippocampus.+However%2C+the+mechanism+behind+this+scenario+has+not+been+fully+understood.%0D%0AWe%2C+for+the+first+time%2C+used+a+roGFP2+based+GSH+sensor+to+monitor+the+OGD-RP+induced+redox+change+of+GSH+system+in+mitochondria+and+cytoplasm+from+3+different+cell+types+(HeLa+cell+culture%2C+pyramidal+cells+and+astrocytes+found+in+OHSC)+in+real-time.+We+observed+that+GSH+in+cytoplasm+was+insensitive+to+OGD-RP%2C+while+noticeable+changes+were+found+in+mitochondria.+Mitochondrial+GSH+get+more+reduced+in+OGD%2C+but+more+oxidized+in+RP%2C+and+that+the+magnitude+of+change+either+in+OGD+or+RP+is+larger+in+pyramidal+cells+than+in+astrocytes%2C+not+significant+in+HeLa+cells.+Heterogeneities+in+ROS+production%2C+antioxidant+capacity+of+GSH+and+mitochondria+membrane+potential+are+responsible+for+the+redox+changes+of+GSH+system+under+contrasting+pathological+conditions%2C+different+cellular+compartments+and+various+cell+types.+%0D%0AIn+order+to+understand+the+differential+susceptibility+to+OGD-RP+in+hippocampal+CA1+and+CA3%2C+we+study+on+ROS+related+critical+molecules+such+NAD(P)H%2C+H2O2+and+GSH.+During+OGD-RP%2C+NAD(P)H+consumption+is+higher+in+CA3+because+it+is+used+more+rapidly+as+reducing+equivalents+to+maintain+larger+antioxidant+pool+in+this+subfield.+Mitochondrial+H2O2+and+the+oxidation+degree+of+mitochondrial+GSH+are+both+lower+in+CA3+during+OGD-RP.+This+can+be+ascribed+to+larger+Trx2+pool+in+CA3+since+Trx2+can+scavenge+H2O2+more+fast+and+efficient+than+GSH+system+and+is+able+to+maintain+lower+H2O2+and+GSH+at+lower+oxidation+degree.&rft.date=2016-01-22&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F26569%2F1%2FETD_BCY_final.pdf&rft.identifier=++Yin%2C+Bocheng++(2016)+Investigation+of+Reactive+Oxygen+Species+Management+in+Biological+and+Pathological+Processes+by+Fluorescence+Imaging.++Doctoral+Dissertation%2C+University+of+Pittsburgh.++++(Unpublished)++