?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F25879%2F&rft.title=Engineering+DNA+Computation+with+Unnatural+Nucleotides+and+Protein+Function+with+Unnatural+Amino+Acids&rft.creator=Prokup%2C+Alexander&rft.description=Synthetic+engineering+methods%2C+such+as+DNA+computation+and+unnatural+amino+acid+mutagenesis%2C+have+provided+a+route+to+improve+the+control+of+DNA+and+proteins.+DNA+computation+encompasses+a+broad+field+that+attempts+to+build+computational+devices+from+DNA+structures.+Logic+gates+are+a+fundamental+component+of+any+larger+computational+network%2C+and+have+been+constructed+from+purely+DNA+frameworks.+Operation+is+determined+by+strict+rules%2C+which+allow+for+the+predictable+creation+of+complex+circuits.+Described+herein+are+methods+to+alter+DNA+logic+gates+with+photochemical+caging+groups%2C+interface+logic+gates+with+protein+output%2C+and+optically+control+DNA+amplification+cycles.+These+methods+have+enabled+precise+temporal+and+spatial+control%2C+as+well+as+merged+the+interface+between+DNA+circuits+and+biological+systems.+Unnatural+amino+acid+mutagenesis+enables+the+site+specific+alteration+of+protein+residues%2C+through+the+insertion+of+a+non-canonical+amino+acid.+Incorporation+of+these+unnatural+residues+has+greatly+expanded+the+function+of+proteins%2C+with+the+introduction+of+new+chemical+functionalities.+These+chemical+handles+have+enabled+applications+such+as+the+study+of+abasic+bypass+in+DNA+polymerases+and+protein-RNA+crosslinking.&rft.date=2015-09-27&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F25879%2F1%2FProkupAM_etd2015.pdf&rft.identifier=++Prokup%2C+Alexander++(2015)+Engineering+DNA+Computation+with+Unnatural+Nucleotides+and+Protein+Function+with+Unnatural+Amino+Acids.++Doctoral+Dissertation%2C+University+of+Pittsburgh.++++(Unpublished)++