?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F24484%2F&rft.title=Validation+of+the+relationship+between+MRCI+polymorphisms+with+dengue+disease+in+a+Brazilian+population&rft.creator=Polglase%2C+Hannah&rft.description=Dengue+is+a+viral+disease+characterized+by+mild+febrile+illness+in+the+majority+of+cases.+In+a+small+percent+of+cases%2C+it+manifests+more+severe+symptoms%2C+and+it+can+be+fatal+without+proper+treatment+and+support.+It+is+a+vector+borne+disease+that+is+transmitted+by+the+Aedes+mosquitos.+Currently+up+to+4+billion+of+the+world%E2%80%99s+population+lives+in+areas+at+risk+for+dengue+transmission.+This+represents+a+public+health+risk.%0D%0AThe+objective+of+this+study+was+to+evaluate+the+association+of+two+single+nucleotide+polymorphisms+(SNPs)+located+in+the+MRCI+gene%2C+rs2296414+and+rs34039386%2C+with+dengue+virus+infection+in+a+sample+of+patients+from+a+cohort+study+of+dengue+patients+and+controls+from+Recife%2C+Brazil.+The+MRCI+gene+codes+for+the+mannose+receptors+of+macrophages%2C+which+have+been+demonstrated+to+play+an+important+role+in+viral+infections.+DNA+was+amplified+using+PCR.+Genotyping+of+179+individuals+was+done+by+three+methods%2C+fluorescence+polarization%2C+restriction+digests%2C+and+sequencing.+All+samples+were+found+to+be+in+Hardy-Weinberg+equilibrium+across+the+sample+population+and+when+classified+as+cases+of+controls.+No+significant+departures+from+Hardy-Weinberg+were+seen+when+samples+were+broken+down+by+disease+status.+Odds+ratios+were+calculated+with+novel+data+and+combined+with+data+from+a+previous+study.+The+previous+study+examined+the+same+two+polymorphisms+using+a+different+sample+from+the+same+cohort+study.++A+statistically+significant+difference+was+found+in+the+combined+samples+for+the+rs2296414+site+with+regards+to+case+or+control+status%2C+OR%3D1.46%2C+p%3D.035.+A+statistically+significant+difference+was+also+found+for+the+rs34039386+site+with+regards+to+case%2Fcontrol+status+OR%3D2.76%2C+p%3D.0029.+This+difference+was+only+found+in+the+novel+data.%0D%0AFurther+research+with+larger+samples+sized+is+needed+to+continue+to+understand+the+relationships+between+these+sites+and+dengue+infection%2C+as+the+sample+size+for+this+study+was+small.+Even+when+combined+with+data+from+the+previous+study%2C+the+data+set+was+not+particularly+large.+The+public+health+significance+of+this+research+is+produced+by+the+increasing+global+importance+of+dengue+and+the+need+for+more+effective+treatments+and+the+development+of+a+vaccine%3B+advancement+of+the+understanding+of+the+relationships+between+the+dengue+virus+and+the+MRCI+gene+may+lead+to+improvements+in+the+treatments+available+and+in+the+production+of+a+vaccine+with+high+efficacy.&rft.date=2015-06-11&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F24484%2F1%2FValidation_of_the_relationship_between_MRCI_polymorphisms_with_dengue_in_a_Brazilain_population_Thesis_HannahPolglase_final.pdf&rft.identifier=++Polglase%2C+Hannah++(2015)+Validation+of+the+relationship+between+MRCI+polymorphisms+with+dengue+disease+in+a+Brazilian+population.++Master's+Thesis%2C+University+of+Pittsburgh.++++(Unpublished)++