eprintid: 24034
rev_number: 26
userid: 4439
dir: disk0/00/02/40/34
datestamp: 2015-05-12 18:45:24
lastmod: 2019-02-02 15:55:11
status_changed: 2015-05-12 18:45:24
type: article
metadata_visibility: show
contact_email: bibbykj@pitt.edu
item_issues_count: 0
eprint_status: archive
creators_name: Mohan, AM
creators_name: Bibby, KJ
creators_name: Lipus, D
creators_name: Hammack, RW
creators_name: Gregory, KB
creators_email: 
creators_email: bibbykj@pitt.edu
creators_email: 
creators_email: 
creators_email: 
creators_id: 
creators_id: BIBBYKJ
creators_id: 
creators_id: 
creators_id: 
contributors_type: http://www.loc.gov/loc.terms/relators/EDT
contributors_name: Forster, Robert J
title: The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing
ispublished: pub
divisions: sch_med_Computational_Systems_Biology
divisions: sch_eng_civilenvironmental
full_text_status: public
abstract: Microbial activity in produced water from hydraulic fracturing operations can lead to undesired environmental impacts and increase gas production costs. However, the metabolic profile of these microbial communities is not well understood. Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing. Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water. The metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, sporulation and dormancy, iron acquisition and metabolism, stress response and sulfur metabolism in the produced water samples. These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.
date: 2014-10-22
date_type: published
publication: PLoS ONE
volume: 9
number: 10
refereed: TRUE
id_number: 10.1371/journal.pone.0107682
other_id: NLM PMC4206270
pmcid: PMC4206270
pmid: 25338024
citation:   Mohan, AM and Bibby, KJ and Lipus, D and Hammack, RW and Gregory, KB  (2014) The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing.  PLoS ONE, 9 (10).       
document_url: http://d-scholarship-dev.library.pitt.edu/24034/1/journal.pone.0107682.pdf
document_url: http://d-scholarship-dev.library.pitt.edu/24034/4/licence.txt