%A J Torres-Kolbus
%A C Chou
%A J Liu
%A A Deiters
%J PLoS ONE
%T Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction
%X Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. ? 2014 Torres-Kolbus et al.
%N 9
%V 9
%D 2014
%R 10.1371/journal.pone.0105467
%L pittir23005