TY  - JOUR
ID  - pittir23005
UR  - http://d-scholarship-dev.library.pitt.edu/23005/
IS  - 9
A1  - Torres-Kolbus, J
A1  - Chou, C
A1  - Liu, J
A1  - Deiters, A
Y1  - 2014/01/01/
N2  - Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. © 2014 Torres-Kolbus et al.
JF  - PLoS ONE
VL  - 9
TI  - Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction
AV  - public
ER  -