<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction"^^ . "Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. © 2014 Torres-Kolbus et al."^^ . "2014-01-01" . . . "9" . "9" . . "PLoS ONE"^^ . . . . . . . . . . . . . . . . . . "Robert W."^^ . "Sobol"^^ . "Robert W. Sobol"^^ . . "A"^^ . "Deiters"^^ . "A Deiters"^^ . . "C"^^ . "Chou"^^ . "C Chou"^^ . . "J"^^ . "Torres-Kolbus"^^ . "J Torres-Kolbus"^^ . . "J"^^ . "Liu"^^ . "J Liu"^^ . . . . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (PDF)"^^ . . . . . . . . "journal.pone.0105467.pdf"^^ . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (Indexer Terms)"^^ . . . . . . "indexcodes.txt"^^ . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (Other)"^^ . . . . . . "small.jpg"^^ . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (Plain Text)"^^ . . . "licence.txt"^^ . . . "Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #23005 \n\nSynthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction\n\n" . "text/html" . .