%0 Journal Article
%A Torres-Kolbus, J
%A Chou, C
%A Liu, J
%A Deiters, A
%D 2014
%F pittir:23005
%J PLoS ONE
%N 9
%T Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction
%U http://d-scholarship-dev.library.pitt.edu/23005/
%V 9
%X Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. © 2014 Torres-Kolbus et al.