@article{pittir23005,
          volume = {9},
          number = {9},
           month = {January},
           title = {Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction},
          author = {J Torres-Kolbus and C Chou and J Liu and A Deiters},
            year = {2014},
         journal = {PLoS ONE},
             url = {http://d-scholarship-dev.library.pitt.edu/23005/},
        abstract = {Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. {\copyright} 2014 Torres-Kolbus et al.}
}