eprintid: 23000
rev_number: 25
userid: 3671
importid: 2826
dir: disk0/00/02/30/00
datestamp: 2014-09-26 17:40:30
lastmod: 2019-02-02 16:58:21
status_changed: 2014-09-26 17:40:29
type: article
metadata_visibility: show
item_issues_count: 0
eprint_status: archive
creators_name: Baron, JL
creators_name: Vikram, A
creators_name: Duda, S
creators_name: Stout, JE
creators_name: Bibby, K
creators_email: 
creators_email: 
creators_email: 
creators_email: jes20@pitt.edu
creators_email: bibbykj@pitt.edu
creators_id: 
creators_id: 
creators_id: 
creators_id: JES20
creators_id: BIBBYKJ
contributors_type: http://www.loc.gov/loc.terms/relators/EDT
contributors_name: Bereswill, Stefan
title: Shift in the microbial ecology of a hospital hot water system following the introduction of an on-site monochloramine disinfection system
ispublished: pub
divisions: sch_gsph_infectiousdiseasesmicrobiology
divisions: sch_med_Computational_Systems_Biology
divisions: sch_eng_civilenvironmental
full_text_status: public
abstract: Drinking water distribution systems, including premise plumbing, contain a diverse microbiological community that may include opportunistic pathogens. On-site supplemental disinfection systems have been proposed as a control method for opportunistic pathogens in premise plumbing. The majority of on-site disinfection systems to date have been installed in hospitals due to the high concentration of opportunistic pathogen susceptible occupants. The installation of on-site supplemental disinfection systems in hospitals allows for evaluation of the impact of on-site disinfection systems on drinking water system microbial ecology prior to widespread application. This study evaluated the impact of supplemental monochloramine on the microbial ecology of a hospital's hot water system. Samples were taken three months and immediately prior to monochloramine treatment and monthly for the first six months of treatment, and all samples were subjected to high throughput Illumina 16S rRNA region sequencing. The microbial community composition of monochloramine treated samples was dramatically different than the baseline months. There was an immediate shift towards decreased relative abundance of Betaproteobacteria, and increased relative abundance of Firmicutes, Alphaproteobacteria, Gammaproteobacteria, Cyanobacteria and Actinobacteria. Following treatment, microbial populations grouped by sampling location rather than sampling time. Over the course of treatment the relative abundance of certain genera containing opportunistic pathogens and genera containing denitrifying bacteria increased. The results demonstrate the driving influence of supplemental disinfection on premise plumbing microbial ecology and suggest the value of further investigation into the overall effects of premise plumbing disinfection strategies on microbial ecology and not solely specific target microorganisms. © 2014 Baron et al.
date: 2014-07-17
date_type: published
publication: PLoS ONE
volume: 9
number: 7
refereed: TRUE
id_number: 10.1371/journal.pone.0102679
citation:   Baron, JL and Vikram, A and Duda, S and Stout, JE and Bibby, K  (2014) Shift in the microbial ecology of a hospital hot water system following the introduction of an on-site monochloramine disinfection system.  PLoS ONE, 9 (7).       
document_url: http://d-scholarship-dev.library.pitt.edu/23000/1/journal.pone.0102679.pdf
document_url: http://d-scholarship-dev.library.pitt.edu/23000/8/licence.txt