eprintid: 22340
rev_number: 25
userid: 1419
dir: disk0/00/02/23/40
datestamp: 2014-07-31 20:45:20
lastmod: 2021-06-13 00:55:55
status_changed: 2014-07-31 20:45:20
type: article
metadata_visibility: show
item_issues_count: 0
eprint_status: archive
creators_name: Epperly, MW
creators_name: Goff, JP
creators_name: Li, S
creators_name: Gao, X
creators_name: Wipf, P
creators_name: Dixon, T
creators_name: Wang, H
creators_name: Franicola, D
creators_name: Shen, H
creators_name: Rwigema, JCM
creators_name: Kagan, V
creators_name: Bernard, M
creators_name: Greenberger, JS
creators_email: 
creators_email: 
creators_email: sol4@pitt.edu
creators_email: 
creators_email: pwipf@pitt.edu
creators_email: 
creators_email: how8@pitt.edu
creators_email: 
creators_email: hos1@pitt.edu
creators_email: 
creators_email: kagan@pitt.edu
creators_email: 
creators_email: joelg@pitt.edu
creators_id: 
creators_id: 
creators_id: SOL4
creators_id: 
creators_id: PWIPF
creators_id: 
creators_id: HOW8
creators_id: 
creators_id: HOS1
creators_id: 
creators_id: KAGAN
creators_id: 
creators_id: JOELG
creators_orcid: 
creators_orcid: 
creators_orcid: 
creators_orcid: 
creators_orcid: 
creators_orcid: 
creators_orcid: 0000-0003-0477-2908
creators_orcid: 
creators_orcid: 
creators_orcid: 
creators_orcid: 
creators_orcid: 
creators_orcid: 
title: Intraesophageal administration of GS-nitroxide (JP4-039) protects against ionizing irradiation-induced esophagitis
ispublished: pub
divisions: sch_as_chemistry
full_text_status: public
abstract: Background/Aim: This study evaluated esophageal radioprotection by the Gramicidin S (GS) derived-nitroxide, JP4-039, a mitochondrial targeting peptide-isostere covalently-linked to 4-amino-Tempo, delivered in a novel swallowed oil-based (F15) formulation. Materials and Methods: C57BL/6HNsd female mice received intraesophageal F15 formulation containing JP4-039 (4 mg/ml in 100 μl volumes) 10 minutes before 28 or 29 Gy upper body irradiation compared to MnSOD-PL (100 μl containing 100 μg plasmid) 24 hours prior to irradiation. Subgroups received 1×107 C57BL/6HNsd, GFP + male bone marrow cells intravenously 5 days after irradiation. Results: JP4-039/F15 or MnSOD-PL increased survival compared to irradiated controls (p<0.0001 for either). Marrow injection further increased survival (p=0.0462 and 0.0351, respectively). Esophagi removed at 1, 3, 7, 14, 24, or 60 days showed bone marrow-derived cells in the esophagi. Conclusion: Intraesophageal GS-nitroxide radioprotection is mediated primarily through recovery of endogenous esophageal progenitor cells.
date: 2010-01-01
date_type: published
publication: In Vivo
volume: 24
number: 6
pagerange: 811 - 819
refereed: TRUE
issn: 0258-851X
other_id: NLM NIHMS426447
other_id: NLM PMC3521523
pmcid: PMC3521523
pmid: 21164038
mesh_headings: Animals
mesh_headings: Bone Marrow Transplantation
mesh_headings: Cytoprotection
mesh_headings: Drug Delivery Systems
mesh_headings: Esophagitis--pathology
mesh_headings: Esophagitis--prevention & control
mesh_headings: Esophagitis--therapy
mesh_headings: Esophagus--drug effects
mesh_headings: Esophagus--pathology
mesh_headings: Esophagus--radiation effects
mesh_headings: Female
mesh_headings: Liposomes--therapeutic use
mesh_headings: Male
mesh_headings: Mice
mesh_headings: Mice, Inbred C57BL
mesh_headings: Nitrogen Oxides--administration & dosage
mesh_headings: Nitrogen Oxides--pharmacokinetics
mesh_headings: Nitrogen Oxides--pharmacology
mesh_headings: Radiation Injuries, Experimental--pathology
mesh_headings: Radiation Injuries, Experimental--prevention & control
mesh_headings: Radiation Injuries, Experimental--therapy
chemical_names: JP4-039
chemical_names: Liposomes
chemical_names: Nitrogen Oxides
citation:   Epperly, MW and Goff, JP and Li, S and Gao, X and Wipf, P and Dixon, T and Wang, H and Franicola, D and Shen, H and Rwigema, JCM and Kagan, V and Bernard, M and Greenberger, JS  (2010) Intraesophageal administration of GS-nitroxide (JP4-039) protects against ionizing irradiation-induced esophagitis.  In Vivo, 24 (6).  811 - 819.  ISSN 0258-851X     
document_url: http://d-scholarship-dev.library.pitt.edu/22340/1/licence.txt