%0 Journal Article
%@ 0258-851X
%A Epperly, MW
%A Goff, JP
%A Li, S
%A Gao, X
%A Wipf, P
%A Dixon, T
%A Wang, H
%A Franicola, D
%A Shen, H
%A Rwigema, JCM
%A Kagan, V
%A Bernard, M
%A Greenberger, JS
%D 2010
%F pittir:22340
%J In Vivo
%N 6
%P 811 - 819
%T Intraesophageal administration of GS-nitroxide (JP4-039) protects against ionizing irradiation-induced esophagitis
%U http://d-scholarship-dev.library.pitt.edu/22340/
%V 24
%X Background/Aim: This study evaluated esophageal radioprotection by the Gramicidin S (GS) derived-nitroxide, JP4-039, a mitochondrial targeting peptide-isostere covalently-linked to 4-amino-Tempo, delivered in a novel swallowed oil-based (F15) formulation. Materials and Methods: C57BL/6HNsd female mice received intraesophageal F15 formulation containing JP4-039 (4 mg/ml in 100 μl volumes) 10 minutes before 28 or 29 Gy upper body irradiation compared to MnSOD-PL (100 μl containing 100 μg plasmid) 24 hours prior to irradiation. Subgroups received 1×107 C57BL/6HNsd, GFP + male bone marrow cells intravenously 5 days after irradiation. Results: JP4-039/F15 or MnSOD-PL increased survival compared to irradiated controls (p<0.0001 for either). Marrow injection further increased survival (p=0.0462 and 0.0351, respectively). Esophagi removed at 1, 3, 7, 14, 24, or 60 days showed bone marrow-derived cells in the esophagi. Conclusion: Intraesophageal GS-nitroxide radioprotection is mediated primarily through recovery of endogenous esophageal progenitor cells.