<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies"^^ . "The translation of in vitro findings to clinical outcomes is often elusive. Trauma/hemorrhagic shock (T/HS) results in hepatic hypoxia that drives inflammation. We hypothesize that in silico methods would help bridge in vitro hepatocyte data and clinical T/HS, in which the liver is a primary site of inflammation. Primary mouse hepatocytes were cultured under hypoxia (1% O 2) or normoxia (21% O2) for 1-72 h, and both the cell supernatants and protein lysates were assayed for 18 inflammatory mediators by Luminex™ technology. Statistical analysis and data-driven modeling were employed to characterize the main components of the cellular response. Statistical analyses, hierarchical and k-means clustering, Principal Component Analysis, and Dynamic Network Analysis suggested MCP-1/CCL2 and IL-1α as central coordinators of hepatocyte-mediated inflammation in C57BL/6 mouse hepatocytes. Hepatocytes from MCP-1-null mice had altered dynamic inflammatory networks. Circulating MCP-1 levels segregated human T/HS survivors from non-survivors. Furthermore, T/HS survivors with elevated early levels of plasma MCP-1 post-injury had longer total lengths of stay, longer intensive care unit lengths of stay, and prolonged requirement for mechanical ventilation vs. those with low plasma MCP-1. This study identifies MCP-1 as a main driver of the response of hepatocytes in vitro and as a biomarker for clinical outcomes in T/HS, and suggests an experimental and computational framework for discovery of novel clinical biomarkers in inflammatory diseases. © 2013 Ziraldo et al."^^ . "2013-12-03" . . . "8" . "12" . . "PLoS ONE"^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "R"^^ . "Zamora"^^ . "R Zamora"^^ . . "Q"^^ . "Mi"^^ . "Q Mi"^^ . . "RA"^^ . "Namas"^^ . "RA Namas"^^ . . "Ioannis P"^^ . "Androulakis"^^ . "Ioannis P Androulakis"^^ . . "Y"^^ . "Vodovotz"^^ . "Y Vodovotz"^^ . . "C"^^ . "Ziraldo"^^ . "C Ziraldo"^^ . . "K"^^ . "Almahmoud"^^ . "K Almahmoud"^^ . . "BS"^^ . "Jefferson"^^ . "BS Jefferson"^^ . . "G"^^ . "Chen"^^ . "G Chen"^^ . . "D"^^ . "Barclay"^^ . "D Barclay"^^ . . "V"^^ . "Tapias"^^ . "V Tapias"^^ . . "TR"^^ . "Billiar"^^ . "TR Billiar"^^ . . . . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (PDF)"^^ . . . . . . . . "Central.pdf"^^ . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (Indexer Terms)"^^ . . . . . . "indexcodes.txt"^^ . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (Other)"^^ . . . . . . "small.jpg"^^ . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (Plain Text)"^^ . . . "licence.txt"^^ . . . "Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #20418 \n\nCentral role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies\n\n" . "text/html" . .