eprintid: 19893
rev_number: 18
userid: 1419
dir: disk0/00/01/98/93
datestamp: 2013-10-28 22:26:22
lastmod: 2019-12-07 15:55:11
status_changed: 2013-10-28 22:26:22
type: article
metadata_visibility: show
item_issues_count: 0
eprint_status: archive
creators_name: Wipf, P
creators_name: Werner, S
creators_name: Twining, LA
creators_name: Kendall, C
creators_email: pwipf@pitt.edu
creators_email: 
creators_email: 
creators_email: 
creators_id: PWIPF
creators_id: 
creators_id: 
creators_id: 
title: HPLC determinations of enantiomeric ratios
ispublished: pub
divisions: sch_as_chemistry
full_text_status: public
abstract: Evaporative light-scattering detection (ELSD) is widely applied in the HPLC analysis of organic compounds lacking a UY chromophore. However, this detection method is generally unsuitable for determination of enantiomeric ratios (er). The er calculated from a UV trace and an ELS trace of the same compound differs significantly. Because of the nonlinear concentration response of the ELS detector, a compound with an er of 95:5 appears to be enantiomerically pure by ELS detection. It is possible to obtain a calibration curve and to calculate a correction factor, but this procedure is time consuming and therefore not very practical for routine analyses. In contrast, a charged aerosol detector allows a very accurate determination of the enantiomeric ratios. Like the ELS detection, the CA detection is independent of the chromophore properties of the substrate. Therefore, we recommend the use of CA instead of ELS detection for determination of the enantiomeric ratios of non-UV active compounds. © 2006 Wiley-Liss, Inc.
date: 2007-01-01
date_type: published
publication: Chirality
volume: 19
number: 1
pagerange: 5 - 9
refereed: TRUE
issn: 0899-0042
id_number: 10.1002/chir.20331
pmid: 17089337
mesh_headings: Chromatography, High Pressure Liquid--methods
mesh_headings: Light
mesh_headings: Scattering, Radiation
mesh_headings: Stereoisomerism
citation:   Wipf, P and Werner, S and Twining, LA and Kendall, C  (2007) HPLC determinations of enantiomeric ratios.  Chirality, 19 (1).  5 - 9.  ISSN 0899-0042     
document_url: http://d-scholarship-dev.library.pitt.edu/19893/1/licence.txt