eprintid: 19204
rev_number: 21
userid: 1346
importid: 1883
dir: disk0/00/01/92/04
datestamp: 2013-07-15 20:17:43
lastmod: 2019-02-02 15:55:27
status_changed: 2013-07-15 20:17:43
type: article
metadata_visibility: show
contact_email: rinaldo@pitt.edu
item_issues_count: 0
eprint_status: archive
creators_name: Kaabinejadian, S
creators_name: Piazza, PA
creators_name: McMurtrey, CP
creators_name: Vernon, SR
creators_name: Cate, SJ
creators_name: Bardet, W
creators_name: Schafer, FB
creators_name: Jackson, KW
creators_name: Campbell, DM
creators_name: Buchli, R
creators_name: Rinaldo, CR
creators_name: Hildebrand, WH
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creators_email: paolo@pitt.edu
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creators_email: RINALDO@pitt.edu
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creators_id: PAOLO
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creators_id: RINALDO
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contributors_type: http://www.loc.gov/loc.terms/relators/EDT
contributors_name: Wang, Tian
title: Identification of Class I HLA T Cell Control Epitopes for West Nile Virus
ispublished: pub
divisions: sch_gsph_infectiousdiseasesmicrobiology
divisions: sch_med_Pathology
full_text_status: public
abstract: The recent West Nile virus (WNV) outbreak in the United States underscores the importance of understanding human immune responses to this pathogen. Via the presentation of viral peptide ligands at the cell surface, class I HLA mediate the T cell recognition and killing of WNV infected cells. At this time, there are two key unknowns in regards to understanding protective T cell immunity: 1) the number of viral ligands presented by the HLA of infected cells, and 2) the distribution of T cell responses to these available HLA/viral complexes. Here, comparative mass spectroscopy was applied to determine the number of WNV peptides presented by the HLA-A*11:01 of infected cells after which T cell responses to these HLA/WNV complexes were assessed. Six viral peptides derived from capsid, NS3, NS4b, and NS5 were presented. When T cells from infected individuals were tested for reactivity to these six viral ligands, polyfunctional T cells were focused on the GTL9 WNV capsid peptide, ligands from NS3, NS4b, and NS5 were less immunogenic, and two ligands were largely inert, demonstrating that class I HLA reduce the WNV polyprotein to a handful of immune targets and that polyfunctional T cells recognize infections by zeroing in on particular HLA/WNV epitopes. Such dominant HLA/peptide epitopes are poised to drive the development of WNV vaccines that elicit protective T cells as well as providing key antigens for immunoassays that establish correlates of viral immunity. © 2013 Kaabinejadian et al.
date: 2013-06-10
date_type: published
publication: PLoS ONE
volume: 8
number: 6
refereed: TRUE
id_number: 10.1371/journal.pone.0066298
citation:   Kaabinejadian, S and Piazza, PA and McMurtrey, CP and Vernon, SR and Cate, SJ and Bardet, W and Schafer, FB and Jackson, KW and Campbell, DM and Buchli, R and Rinaldo, CR and Hildebrand, WH  (2013) Identification of Class I HLA T Cell Control Epitopes for West Nile Virus.  PLoS ONE, 8 (6).       
document_url: http://d-scholarship-dev.library.pitt.edu/19204/1/Identification_of_Class_I_HLA_T_Cell_Control_Epitopes_for_West_Nile_Virus.pdf
document_url: http://d-scholarship-dev.library.pitt.edu/19204/8/licence.txt