eprintid: 18777
rev_number: 16
userid: 1419
dir: disk0/00/01/87/77
datestamp: 2013-05-28 15:30:41
lastmod: 2021-04-11 08:55:15
status_changed: 2013-05-28 15:30:41
type: article
metadata_visibility: show
item_issues_count: 0
eprint_status: archive
creators_name: Gokhale, A
creators_name: Rwigema, JC
creators_name: Epperly, MW
creators_name: Glowacki, J
creators_name: Wang, H
creators_name: Wipf, P
creators_name: Goff, JP
creators_name: Dixon, T
creators_name: Patrene, K
creators_name: Greenberger, JS
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creators_email: how8@pitt.edu
creators_email: pwipf@pitt.edu
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creators_email: joelg@pitt.edu
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creators_id: HOW8
creators_id: PWIPF
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creators_id: JOELG
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creators_orcid: 0000-0003-0477-2908
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title: Small molecule GS-nitroxide ameliorates ionizing irradiation-induced delay in bone wound healing in a novel murine model
ispublished: pub
divisions: sch_as_chemistry
full_text_status: public
abstract: We studied radioprotection and mitigation by mitochondrial-targeted Tempol (GS-nitroxide, JP4-039), in a mouse injury/irradiation model of combined injury (fracture/irradiation). Right hind legs of control C57BL/6NHsd female mice, mice pretreated with MnSOD-PL, JP4-039, or with amifostine were irradiated with single and fractionated doses of 0 to 20 Gy. Twenty-four hours later, unicortical holes were drilled into the tibiae of both hind legs; at intervals, tibias were excised, radiographed, and processed for histology. Bone wounds irradiated to 20 or 30 Gy showed delayed healing at 21 to 28 days. Treatment with JP4-039 MnSOD-PL or amifostine, before or after single fraction 20 Gy or during fractionated irradiation followed by drilling accelerated wound healing at days 21 and 28. Orthotopic 3LL tumors were not protected by JP4-039 or amifostine. In nonirradiated mice, pretreatment with JP4-039 accelerated bone wound healing. This test system should be useful for the development of new small molecule radioprotectors.
date: 2010-01-01
date_type: published
publication: In Vivo
volume: 24
number: 4
pagerange: 377 - 385
refereed: TRUE
issn: 0258-851X
other_id: NLM NIHMS223059
other_id: NLM PMC2916688
pmcid: PMC2916688
pmid: 20668303
mesh_headings: Animals
mesh_headings: Cyclic N-Oxides--therapeutic use
mesh_headings: Disease Models, Animal
mesh_headings: Dose-Response Relationship, Radiation
mesh_headings: Female
mesh_headings: Hindlimb
mesh_headings: Mice
mesh_headings: Mice, Inbred C57BL
mesh_headings: Radiation Injuries--drug therapy
mesh_headings: Radiation Injuries--prevention & control
mesh_headings: Radiation, Ionizing
mesh_headings: Radiation-Protective Agents--therapeutic use
mesh_headings: Spin Labels
mesh_headings: Tibia--drug effects
mesh_headings: Tibia--injuries
mesh_headings: Tibia--radiography
mesh_headings: Wound Healing--radiation effects
chemical_names: Cyclic N-Oxides
chemical_names: Radiation-Protective Agents
chemical_names: Spin Labels
chemical_names: tempol
citation:   Gokhale, A and Rwigema, JC and Epperly, MW and Glowacki, J and Wang, H and Wipf, P and Goff, JP and Dixon, T and Patrene, K and Greenberger, JS  (2010) Small molecule GS-nitroxide ameliorates ionizing irradiation-induced delay in bone wound healing in a novel murine model.  In Vivo, 24 (4).  377 - 385.  ISSN 0258-851X     
document_url: http://d-scholarship-dev.library.pitt.edu/18777/1/licence.txt