eprintid: 17872 rev_number: 19 userid: 1346 importid: 1604 dir: disk0/00/01/78/72 datestamp: 2013-03-28 16:47:13 lastmod: 2019-02-04 15:58:26 status_changed: 2013-03-28 16:47:13 type: article metadata_visibility: show contact_email: shanksrm@upmc.edu item_issues_count: 0 eprint_status: archive creators_name: Shanks, RMQ creators_name: Lahr, RM creators_name: Stella, NA creators_name: Arena, KE creators_name: Brothers, KM creators_name: Kwak, DH creators_name: Liu, X creators_name: Kalivoda, EJ creators_email: creators_email: rml40@pitt.edu creators_email: nas92@pitt.edu creators_email: creators_email: kmb227@pitt.edu creators_email: dhk19@pitt.edu creators_email: xinyuliu@pitt.edu creators_email: creators_id: creators_id: RML40 creators_id: NAS92 creators_id: creators_id: KMB227 creators_id: DHK19 creators_id: XINYULIU creators_id: creators_orcid: creators_orcid: 0000-0002-7469-4565 creators_orcid: creators_orcid: creators_orcid: creators_orcid: creators_orcid: creators_orcid: title: A Serratia marcescens PigP Homolog Controls Prodigiosin Biosynthesis, Swarming Motility and Hemolysis and Is Regulated by cAMP-CRP and HexS ispublished: pub divisions: sch_as_chemistry divisions: sch_med_Ophthalmology full_text_status: public abstract: Swarming motility and hemolysis are virulence-associated determinants for a wide array of pathogenic bacteria. The broad host-range opportunistic pathogen Serratia marcescens produces serratamolide, a small cyclic amino-lipid, that promotes swarming motility and hemolysis. Serratamolide is negatively regulated by the transcription factors HexS and CRP. Positive regulators of serratamolide production are unknown. Similar to serratamolide, the antibiotic pigment, prodigiosin, is regulated by temperature, growth phase, HexS, and CRP. Because of this co-regulation, we tested the hypothesis that a homolog of the PigP transcription factor of the atypical Serratia species ATCC 39006, which positively regulates prodigiosin biosynthesis, is also a positive regulator of serratamolide production in S. marcescens. Mutation of pigP in clinical, environmental, and laboratory strains of S. marcescens conferred pleiotropic phenotypes including the loss of swarming motility, hemolysis, and severely reduced prodigiosin and serratamolide synthesis. Transcriptional analysis and electrophoretic mobility shift assays place PigP in a regulatory pathway with upstream regulators CRP and HexS. The data from this study identifies a positive regulator of serratamolide production, describes novel roles for the PigP transcription factor, shows for the first time that PigP directly regulates the pigment biosynthetic operon, and identifies upstream regulators of pigP. This study suggests that PigP is important for the ability of S. marcescens to compete in the environment. © 2013 Shanks et al. date: 2013-03-01 date_type: published publication: PLoS ONE volume: 8 number: 3 refereed: TRUE id_number: 10.1371/journal.pone.0057634 other_id: NLM PMC3585978 pmcid: PMC3585978 pmid: 23469212 citation: Shanks, RMQ and Lahr, RM and Stella, NA and Arena, KE and Brothers, KM and Kwak, DH and Liu, X and Kalivoda, EJ (2013) A Serratia marcescens PigP Homolog Controls Prodigiosin Biosynthesis, Swarming Motility and Hemolysis and Is Regulated by cAMP-CRP and HexS. PLoS ONE, 8 (3). document_url: http://d-scholarship-dev.library.pitt.edu/17872/1/A_Serratia_marcescens.pdf document_url: http://d-scholarship-dev.library.pitt.edu/17872/8/licence.txt