eprintid: 17797
rev_number: 25
userid: 1419
dir: disk0/00/01/77/97
datestamp: 2013-03-20 16:43:18
lastmod: 2021-06-12 23:55:32
status_changed: 2013-03-20 16:43:18
type: article
metadata_visibility: show
item_issues_count: 0
eprint_status: archive
creators_name: Ozdemir, A
creators_name: Lednev, IK
creators_name: Asher, SA
creators_email: 
creators_email: 
creators_email: asher@pitt.edu
creators_id: 
creators_id: 
creators_id: ASHER
title: Comparison between UV Raman and circular dichroism detection of short α helices in bombolitin III
ispublished: pub
divisions: sch_as_chemistry
full_text_status: public
abstract: We have used UV resonance Raman (UVRR) and circular dichroism (CD) spectroscopies to examine the dilute solution-phase secondary structure of the 17 amino acid peptide Bombolitin III (BIII). Both UVRR and CD clearly observe the α-helix structure induced by the addition of trifluoroethanol (TFE) to BIII. In contrast, only UVRR is able to detect the single α-helical turn induced by increasing the pH of BIII from pH 1.8 to 6.4. This α-helical turn is formed because of a stabilizing salt bridge formed between Lys2 and Asp5. Further increases in the α-helix content occur as the pH is raised further. We compare the relative sensitivity of UVRR and CD to short α helices and find, as expected, that the CD cannot detect short α helices. This study demonstrates that UV Raman measurements can detect the formation of single α-helical turns which cannot be detected by CD measurements.
date: 2002-02-12
date_type: published
publication: Biochemistry
volume: 41
number: 6
pagerange: 1893 - 1896
refereed: TRUE
issn: 0006-2960
id_number: 10.1021/bi010970e
pmid: 11827535
mesh_headings: Amino Acid Sequence
mesh_headings: Circular Dichroism
mesh_headings: Molecular Sequence Data
mesh_headings: Peptides--chemistry
mesh_headings: Protein Structure, Secondary
mesh_headings: Spectrum Analysis, Raman
chemical_names: Peptides
chemical_names: bombolitins
citation:   Ozdemir, A and Lednev, IK and Asher, SA  (2002) Comparison between UV Raman and circular dichroism detection of short α helices in bombolitin III.  Biochemistry, 41 (6).  1893 - 1896.  ISSN 0006-2960     
document_url: http://d-scholarship-dev.library.pitt.edu/17797/1/licence.txt