relation: http://d-scholarship-dev.library.pitt.edu/17797/
title: Comparison between UV Raman and circular dichroism detection of short α helices in bombolitin III
creator: Ozdemir, A
creator: Lednev, IK
creator: Asher, SA
description: We have used UV resonance Raman (UVRR) and circular dichroism (CD) spectroscopies to examine the dilute solution-phase secondary structure of the 17 amino acid peptide Bombolitin III (BIII). Both UVRR and CD clearly observe the α-helix structure induced by the addition of trifluoroethanol (TFE) to BIII. In contrast, only UVRR is able to detect the single α-helical turn induced by increasing the pH of BIII from pH 1.8 to 6.4. This α-helical turn is formed because of a stabilizing salt bridge formed between Lys2 and Asp5. Further increases in the α-helix content occur as the pH is raised further. We compare the relative sensitivity of UVRR and CD to short α helices and find, as expected, that the CD cannot detect short α helices. This study demonstrates that UV Raman measurements can detect the formation of single α-helical turns which cannot be detected by CD measurements.
date: 2002-02-12
type: Article
type: PeerReviewed
format: text/plain
language: en
rights: attached
identifier: http://d-scholarship-dev.library.pitt.edu/17797/1/licence.txt
identifier:   Ozdemir, A and Lednev, IK and Asher, SA  (2002) Comparison between UV Raman and circular dichroism detection of short α helices in bombolitin III.  Biochemistry, 41 (6).  1893 - 1896.  ISSN 0006-2960     
relation: 10.1021/bi010970e