@article{pittir17797,
          volume = {41},
          number = {6},
           month = {February},
          author = {A Ozdemir and IK Lednev and SA Asher},
           title = {Comparison between UV Raman and circular dichroism detection of short {\ensuremath{\alpha}} helices in bombolitin III},
         journal = {Biochemistry},
           pages = {1893 -- 1896},
            year = {2002},
             url = {http://d-scholarship-dev.library.pitt.edu/17797/},
        abstract = {We have used UV resonance Raman (UVRR) and circular dichroism (CD) spectroscopies to examine the dilute solution-phase secondary structure of the 17 amino acid peptide Bombolitin III (BIII). Both UVRR and CD clearly observe the {\ensuremath{\alpha}}-helix structure induced by the addition of trifluoroethanol (TFE) to BIII. In contrast, only UVRR is able to detect the single {\ensuremath{\alpha}}-helical turn induced by increasing the pH of BIII from pH 1.8 to 6.4. This {\ensuremath{\alpha}}-helical turn is formed because of a stabilizing salt bridge formed between Lys2 and Asp5. Further increases in the {\ensuremath{\alpha}}-helix content occur as the pH is raised further. We compare the relative sensitivity of UVRR and CD to short {\ensuremath{\alpha}} helices and find, as expected, that the CD cannot detect short {\ensuremath{\alpha}} helices. This study demonstrates that UV Raman measurements can detect the formation of single {\ensuremath{\alpha}}-helical turns which cannot be detected by CD measurements.}
}