TY  - JOUR
ID  - pittir17336
UR  - http://d-scholarship-dev.library.pitt.edu/17336/
IS  - 3
A1  - Baca, JT
A1  - Finegold, DN
A1  - Asher, SA
Y1  - 2008/01/01/
N2  - The difficulty of rapid, definitive diagnosis of myocardial ischemia leads to unnecessary hospital admissions and treatment delays. Previously, decreased metal binding affinity in human serum was investigated as a marker for myocardial ischemia. Polymerized Crystalline Colloidal Array (PCCA) sensors for Ni2+ may be useful in developing a point-of-care test to determine metal binding affinity in plasma and to help rule out myocardial ischemia. PCCA sensors for Ni2+, with 5-amino-8-hydroxyquinoline as a chelating agent, were tested in aqueous solutions and diluted human plasma. The peak wavelength diffracted by the sensors was monitored by reflectance spectrometry and correlated with Ni2+ concentration. The PCCA sensors show a linear response to aqueous Ni2+ concentrations between 0.2 and 1.0 mmol L-1, and can detect changes in free Ni2+ concentration of <60 ?mol L-1. The sensors respond at physiologic pH and can be reversibly dehydrated. The PCCA sensors developed here can report on free Ni2+ concentration in the presence of human plasma. These sensors can be used to detect a decrease in the Ni2+ affinity of plasma proteins, which may indicate recent myocardial ischemia. PCCA sensors offer a practical approach to rapid, point-of-care detection of a proposed biochemical signature of myocardial ischemia. © The Royal Society of Chemistry.
JF  - Analyst
VL  - 133
SN  - 0003-2654
TI  - Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia
SP  - 385 
AV  - public
EP  -  390
ER  -