eprintid: 17246
rev_number: 22
userid: 1419
dir: disk0/00/01/72/46
datestamp: 2013-02-08 20:56:31
lastmod: 2021-06-12 23:55:37
status_changed: 2013-02-08 20:56:31
type: article
metadata_visibility: show
item_issues_count: 0
eprint_status: archive
creators_name: Xiong, K
creators_name: Asher, SA
creators_email: 
creators_email: asher@pitt.edu
creators_id: 
creators_id: ASHER
title: Circular dichroism and UV resonance raman study of the impact of alcohols on the gibbs free energy landscape of an α-helical peptide
ispublished: pub
divisions: sch_as_chemistry
full_text_status: public
abstract: We used CD and UV resonance Raman spectroscopy to study the impact of alcohols on the conformational equilibria and relative Gibbs free energy landscapes along the Ramachandran φ-coordinate of a mainly poly-Ala peptide, AP with an AAAAA(AAARA)3A sequence. 2,2,2-Trifluoroethanol (TFE) most stabilizes the α-helix-like conformations, followed by ethanol, methanol, and pure water. The π-bulge conformation is stabilized more than the α-helix, while the 310-helix is destabilized due to the alcohol-increased hydrophobicity. Turns are also stabilized by alcohols. We also found that while TFE induces more α-helices, it favors multiple, shorter helix segments. © 2010 American Chemical Society.
date: 2010-04-20
date_type: published
publication: Biochemistry
volume: 49
number: 15
pagerange: 3336 - 3342
refereed: TRUE
issn: 0006-2960
id_number: 10.1021/bi100176a
other_id: NLM NIHMS188980
other_id: NLM PMC2857330
pmcid: PMC2857330
pmid: 20225890
mesh_headings: Alcohols--pharmacology
mesh_headings: Amino Acid Sequence
mesh_headings: Circular Dichroism
mesh_headings: Ethanol--pharmacology
mesh_headings: Indicators and Reagents
mesh_headings: Methanol--pharmacology
mesh_headings: Models, Molecular
mesh_headings: Peptides--chemistry
mesh_headings: Peptides--drug effects
mesh_headings: Protein Conformation
mesh_headings: Spectrophotometry, Ultraviolet
mesh_headings: Spectrum Analysis, Raman
mesh_headings: Thermodynamics
mesh_headings: Water--chemistry
chemical_names: Alcohols
chemical_names: Indicators and Reagents
chemical_names: Peptides
chemical_names: Ethanol
chemical_names: Methanol
chemical_names: Water
citation:   Xiong, K and Asher, SA  (2010) Circular dichroism and UV resonance raman study of the impact of alcohols on the gibbs free energy landscape of an α-helical peptide.  Biochemistry, 49 (15).  3336 - 3342.  ISSN 0006-2960     
document_url: http://d-scholarship-dev.library.pitt.edu/17246/1/licence.txt