?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F17191%2F&rft.title=Chikungunya+Virus+Glycoproteins+Mediate+Viral+Entry+and+Cellular+Fusion&rft.creator=Rinchuse%2C+Derek&rft.description=The+Chikungunya+virus+(CHIKV)+has+currently+been+identified+in+over+40+countries+and+in+2008+was+listed+as+a+US+National+Institute+of+Allergy+and+Infectious+Diseases+(NIAID)+category+C+priority+pathogen.+Outbreaks+of+the+virus+have+been+documented+as+early+as+1779+and+frequent+outbreaks+have+been+reported+through+1960-2003%2C+most+notably+in+Reunion+Island%2C+a+French+overseas+department+in+the+Indian+Ocean.+Out+of+a+total+population+of+785%2C000+in+Reunion+Island%2C+300%2C000+cases+were+reported+including+a+total+of+237+deaths.+Numerous+aspects+of+the+viral+life+cycle+are+unknown%2C+with+no+current+vaccine+the+implementation+of+more+research+and+dissemination+of+more+knowledge+is+of+great+public+health+importance.+A+CHIKV+construct+was+synthesized+by+Genewiz%2C+containing+CHIKV+structural+proteins+in+pcDNA+3.1.++This+construct+was+used+to+create+CHIKV+pseudo-viral+particles+with+a+luciferase+based+reporter.++The+pseudo-virus+was+used+to+survey+many+cell+lines+for+permissivity+to+infection.++This+construct+was+also+used+to+create+3+other+constructs+containing+CHIKV+E1%2C+E2%2C+and+E3+individually.++These+constructs+were+used+individually+and+in+combination+with+each+other+to+create+pseudo-viruses+for+cellular+infection.++The+cell-cell+fusion+capabilities+of+the+full+CHIKV+construct+along+with+the+individual+envelope+proteins+were+also+tested+in+a+Cre-Lox+system.&rft.date=2013-01-29&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F17191%2F1%2FRinchuse_Derek_MSThesisFinal.pdf&rft.identifier=++Rinchuse%2C+Derek++(2013)+Chikungunya+Virus+Glycoproteins+Mediate+Viral+Entry+and+Cellular+Fusion.++Master's+Thesis%2C+University+of+Pittsburgh.++++(Unpublished)++