?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F17141%2F&rft.title=Carbon+Nanomaterial-Based+Biosensing&rft.creator=Chen%2C+Yanan&rft.description=Carbon+nanomaterials%2C+including+carbon+nanotubes+(CNTs)+and+graphene%2C+are+exciting+materials+that+have+been+the+focus+of+research+in+recent+years.+They+have+unique+physical+and+chemical+properties+such+as+high+carrier+mobility%2C+high+surface-to-volume+ratio%2C+and+robustness%2C+and+are+thus+considered+to+be+ideal+candidates+to+interact+with+biological+systems.+However%2C+the+pristine+carbon+nanomaterials+usually+suffer+from+the+lack+of+solubility+in+aqueous+systems.+Furthermore%2C+these+materials+are+extremely+sensitive+to+any+change+in+their+immediate+environment%2C+so+it+is+necessary+to+decorate+these+materials+with+receptor+molecules+to+provide+specificity.+Therefore+the+chemical+functionalizations+of+carbon+nanomaterials+are+important+before+such+materials+can+be+incorporated+into+biosensing+devices.+There+are+commonly+two+approaches+of+functionalizing+carbon+nanomaterials%2C+namely+covalent+and+noncovalent+methods.+Covalent+functionalization+can+provide+robust+chemical+groups+onto+the+surface+of+carbon+nanomaterials.+However%2C+this+method+may+disrupt+the+sp2+structure+of+carbon+nanomaterials+and+affect+the+electronic+properties+of+these+materials.+Noncovalent+functionalization+can+bring+the+desired+functionalization+while+maintaining+the+intrinsic+electronic+properties+intact%2C+but+leaching+of+the+absorbed+materials+may+be+a+problem.+%0D%0AIn+this+dissertation%2C+both+methods+have+been+used+to+functionalize+carbon+nanomaterials+towards+biosensors.+Using+noncovalent+functionalization+scheme%2C+we+built+two+sensing+platforms.+We+used+epigallocatechin+gallate+(EGCG)+to+noncovalently+attach+to+the+surface+of+single-walled+carbon+nanotubes+(SWNT)+and+fabricated+a+resistor-based+sensor+for+hydrogen+peroxide.+Using+porphyrin-based+glycoconjugate%2C+we+explored+the+sensitivity+of+SWNT%2Fglycoconjugate+composites+towards+lectins.+Lectins+are+sugar-binding+proteins+that+exist+on+the+surface+of+bacteria.+Sensing+of+lectins+may+lead+to+the+development+of+bacteria+detectors.+Furthermore%2C+to+explore+an+optimized+system+for+lectin+sensing%2C+we+used+SWNTs+and+graphene%2C+as+well+as+different+glycoconjugates.+It+was+found+out+that+SWNTs+performed+better+than+chemically+converted+graphene+(CCG).+Although+CCG+was+not+suitable+for+sensing+of+lectins+using+noncovalent+functionalization%2C+holey+reduced+graphene+oxide+(hRGO)%2C+with+its+interconnected+graphene+nanoribbon+structure+and+abundant+oxygen-containing+groups%2C+may+be+a+good+platform+for+biosensing.+The+functionalization+of+these+groups+with+antimicrobial+peptide+can+provide+specificity+to+certain+bacteria%2C+towards+the+fabrication+of+a+bacteria+detector.+%0D%0A&rft.date=2013-07-24&rft.type=University+of+Pittsburgh+ETD&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fd-scholarship-dev.library.pitt.edu%2F17141%2F1%2FDissertation_YananChen_May20_2013.pdf&rft.identifier=++Chen%2C+Yanan++(2013)+Carbon+Nanomaterial-Based+Biosensing.++Doctoral+Dissertation%2C+University+of+Pittsburgh.++++(Unpublished)++