eprintid: 16737
rev_number: 23
userid: 1586
dir: disk0/00/01/67/37
datestamp: 2013-01-25 14:18:26
lastmod: 2018-01-25 06:15:03
status_changed: 2013-01-25 14:18:26
type: thesis_degree
metadata_visibility: show
contact_email: marshall.mcgoff@gmail.com
item_issues_count: 0
eprint_status: archive
creators_name: McGoff, Marshall
creators_email: msm73@pitt.edu
creators_id: MSM73
title: CYSTEINE AND SPIN LABEL MODIFICATION AS A MEANS TO EXPLORE PROTEIN DYNAMICS AND STRUCTURE
ispublished: unpub
divisions: sch_as_chemistry
full_text_status: public
keywords: ESR, MSH, GB1
abstract: The goal of this work is to expand upon the current labeling technology available in the field of site directed spin labeling (SDSL) by cysteine and spin label modification. Multiple mutants of the B1 immunoglobulin-binding domain of protein G (GB1) were expressed, purified, characterized, and described herein. The synthesis and full characterization is described for several initial compounds along the pathway to a more rigid linker with less orientational freedom. The modification of cysteine using O-mesitylenesulfonylhydroxylamine (MSH) to form dehydroalanine with subsequent attachment of a thiol is detailed in this work. Electron Spin Resonance (ESR) spectra were collected as a means for comparison to newly designed labels. Future directions for the project include the completion of the synthesis and incorporation of new spin labels using dehydroalanine.
date: 2013-01-25
date_type: published
pages: 70
institution: University of Pittsburgh
refereed: TRUE
etdcommittee_type: committee_chair
etdcommittee_type: committee_member
etdcommittee_type: committee_member
etdcommittee_name: Saxena, Sunil
etdcommittee_name: Horne, W. Seth
etdcommittee_name: Garrett-Roe, Sean
etdcommittee_email: sksaxena@pitt.edu
etdcommittee_email: horne@pitt.edu
etdcommittee_email: sgr@pitt.edu
etdcommittee_id: SKSAXENA
etdcommittee_id: HORNE
etdcommittee_id: SGR
etd_defense_date: 2012-11-19
etd_approval_date: 2013-01-25
etd_submission_date: 2012-12-03
etd_release_date: 2013-01-25
etd_access_restriction: 5_year
etd_patent_pending: FALSE
thesis_type: thesis
degree: MS
citation:   McGoff, Marshall  (2013) CYSTEINE AND SPIN LABEL MODIFICATION AS A MEANS TO EXPLORE PROTEIN DYNAMICS AND STRUCTURE.  Master's Thesis, University of Pittsburgh.    (Unpublished)  
document_url: http://d-scholarship-dev.library.pitt.edu/16737/7/msm73_thesis.pdf