eprintid: 15568
rev_number: 22
userid: 1346
importid: 758
dir: disk0/00/01/55/68
datestamp: 2012-10-04 14:14:38
lastmod: 2019-02-04 15:58:27
status_changed: 2012-10-04 14:14:38
type: article
metadata_visibility: show
contact_email: shanksrm@upmc.edu
item_issues_count: 0
eprint_status: archive
creators_name: Shanks, RMQ
creators_name: Stella, NA
creators_name: Lahr, RM
creators_name: Wang, S
creators_name: Veverka, TI
creators_name: Kowalski, RP
creators_name: Liu, X
creators_email: rms68@pitt.edu
creators_email: nas92@pitt.edu
creators_email: rml40@pitt.edu
creators_email: 
creators_email: 
creators_email: kowalsk@pitt.edu
creators_email: xinyuliu@pitt.edu
creators_id: RMS68
creators_id: NAS92
creators_id: RML40
creators_id: 
creators_id: 
creators_id: KOWALSK
creators_id: XINYULIU
creators_orcid: 
creators_orcid: 
creators_orcid: 0000-0002-7469-4565
creators_orcid: 
creators_orcid: 
creators_orcid: 0000-0002-8558-0782
creators_orcid: 
contributors_type: http://www.loc.gov/loc.terms/relators/EDT
contributors_name: Webber, Mark Alexander
title: Serratamolide is a hemolytic factor produced by Serratia marcescens
ispublished: pub
divisions: sch_as_chemistry
divisions: sch_med_Ophthalmology
full_text_status: public
abstract: Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use. © 2012 Shanks et al.
date: 2012-05-16
date_type: published
publication: PLoS ONE
volume: 7
number: 5
refereed: TRUE
id_number: 10.1371/journal.pone.0036398
other_id: NLM PMC3353980
pmcid: PMC3353980
pmid: 22615766
mesh_headings: Contact Lenses--microbiology
mesh_headings: Depsipeptides--pharmacology
mesh_headings: Genes, Bacterial
mesh_headings: Genetic Complementation Test
mesh_headings: Hemolysis--drug effects
mesh_headings: Mutation
mesh_headings: Serratia marcescens--genetics
mesh_headings: Serratia marcescens--isolation & purification
mesh_headings: Serratia marcescens--metabolism
chemical_names: Depsipeptides
chemical_names: serratamolide
citation:   Shanks, RMQ and Stella, NA and Lahr, RM and Wang, S and Veverka, TI and Kowalski, RP and Liu, X  (2012) Serratamolide is a hemolytic factor produced by Serratia marcescens.  PLoS ONE, 7 (5).       
document_url: http://d-scholarship-dev.library.pitt.edu/15568/1/Serratamolide.pdf
document_url: http://d-scholarship-dev.library.pitt.edu/15568/8/licence.txt