eprintid: 14442
rev_number: 13
userid: 1291
dir: disk0/00/01/44/42
datestamp: 2012-09-25 14:22:29
lastmod: 2017-12-02 16:55:34
status_changed: 2012-09-25 14:22:29
type: article
metadata_visibility: show
item_issues_count: 0
eprint_status: archive
creators_name: Holtz, JS
creators_name: Lednev, IK
creators_name: Asher, SA
creators_email: 
creators_email: 
creators_email: asher@pitt.edu
creators_id: 
creators_id: 
creators_id: ASHER
title: UV resonance Raman study of angiotensin II conformation in nonaqueous environments: lipid micelles and acetonitrile.
ispublished: pub
divisions: sch_as_chemistry
full_text_status: public
keywords: Acetonitriles, Angiotensin II, Animals, Humans, Lipids, Membranes, Artificial, Micelles, Phosphorylcholine, Protein Conformation, Protein Structure, Secondary, Sodium Dodecyl Sulfate, Solvents, Spectrum Analysis, Raman, Ultraviolet Rays, Water
abstract: We used 206.5-nm excited resonance Raman measurements to examine the angiotensin II (AII) secondary structure in H(2)O in the presence of dodecylphosphocholine (DPC) micelles, sodium dodecylsulfate (SDS) monomers and micelles, and in a 70% acetonitrile (ACN-d)-30% water solution. Our AII-SDS titration absorption studies indicate the formation of a 1:2 AII:SDS complex in which two negatively charged SDS molecules attach to the AII positively charged N terminus and to Arg(2). Our 206.5-nm excited Raman results indicate that the 1:2 AII:SDS complexation increases the AII beta-turn composition. We also used 228.9-nm Raman excitation to probe the local solvent accessibility of Tyr(4) (AII) in DPC and SDS micelles. Our Tyr (AII) solvent accessibility studies suggest that the Tyr residue is more exposed to the aqueous environment in SDS micelles than in DPC micelles.
date: 2000
date_type: published
publication: Biopolymers
volume: 57
number: 2
pagerange: 55 - 63
event_location: United States
refereed: TRUE
issn: 0006-3525
funders: NIGMS NIH HHS (R01GM30741)
id_number: 10.1002/(SICI)1097-0282(2000)57:2<55::AID-BIP2>3.0.CO;2-B
other_id: 10.1002/(SICI)1097-0282(2000)57:2<55::AID-BIP2>3.0.CO;2-B
pmid: 10766956
mesh_headings: Acetonitriles--pharmacology
mesh_headings: Angiotensin II--chemistry
mesh_headings: Angiotensin II--radiation effects
mesh_headings: Animals
mesh_headings: Humans
mesh_headings: Lipids--chemistry
mesh_headings: Lipids--pharmacology
mesh_headings: Membranes, Artificial
mesh_headings: Micelles
mesh_headings: Phosphorylcholine--analogs & derivatives
mesh_headings: Phosphorylcholine--chemistry
mesh_headings: Phosphorylcholine--pharmacology
mesh_headings: Protein Conformation--drug effects
mesh_headings: Protein Structure, Secondary--drug effects
mesh_headings: Sodium Dodecyl Sulfate--chemistry
mesh_headings: Sodium Dodecyl Sulfate--pharmacology
mesh_headings: Solvents--pharmacology
mesh_headings: Spectrum Analysis, Raman--methods
mesh_headings: Ultraviolet Rays
mesh_headings: Water--pharmacology
chemical_names: Acetonitriles
chemical_names: Lipids
chemical_names: Membranes, Artificial
chemical_names: Micelles
chemical_names: Solvents
chemical_names: Phosphorylcholine
chemical_names: Angiotensin II
chemical_names: Sodium Dodecyl Sulfate
chemical_names: dodecylphosphocholine
chemical_names: acetonitrile
chemical_names: Water
language: eng
citation:   Holtz, JS and Lednev, IK and Asher, SA  (2000) UV resonance Raman study of angiotensin II conformation in nonaqueous environments: lipid micelles and acetonitrile.  Biopolymers, 57 (2).  55 - 63.  ISSN 0006-3525     
document_url: http://d-scholarship-dev.library.pitt.edu/14442/1/licence.txt