eprintid: 14156 rev_number: 19 userid: 1346 importid: 661 dir: disk0/00/01/41/56 datestamp: 2012-09-13 20:24:31 lastmod: 2019-02-04 15:56:41 status_changed: 2012-09-13 20:24:31 type: article metadata_visibility: show contact_email: rprevots@niaid.nih.gov item_issues_count: 0 eprint_status: archive creators_name: de Filippis, I creators_name: de Lemos, APS creators_name: Hostetler, JB creators_name: Wollenberg, K creators_name: Sacchi, CT creators_name: Harrison, LH creators_name: Bash, MC creators_name: Prevots, DR creators_email: creators_email: creators_email: creators_email: creators_email: creators_email: lharriso@edc.pitt.edu creators_email: creators_email: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: LHARRISO creators_id: creators_id: contributors_type: http://www.loc.gov/loc.terms/relators/EDT contributors_name: Borrow, Ray title: Molecular epidemiology of neisseria meningitidis serogroup B in Brazil ispublished: pub divisions: sch_gsph_infectiousdiseasesmicrobiology full_text_status: public abstract: Background: Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of São Paulo (1988-2006) for study (n = 372). Methods: We performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA. Results: In 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the São Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1. Conclusions: A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp. date: 2012-03-14 date_type: published publication: PLoS ONE volume: 7 number: 3 refereed: TRUE id_number: 10.1371/journal.pone.0033016 other_id: NLM PMC3303791 pmcid: PMC3303791 pmid: 22431994 mesh_headings: Bacterial Typing Techniques mesh_headings: Base Sequence mesh_headings: Biodiversity mesh_headings: Brazil--epidemiology mesh_headings: Genes, Bacterial--genetics mesh_headings: Genetic Variation mesh_headings: Geography mesh_headings: Humans mesh_headings: Likelihood Functions mesh_headings: Meningococcal Infections--epidemiology mesh_headings: Meningococcal Infections--genetics mesh_headings: Meningococcal Infections--microbiology mesh_headings: Molecular Epidemiology mesh_headings: Molecular Sequence Data mesh_headings: Multilocus Sequence Typing mesh_headings: Neisseria meningitidis, Serogroup B--classification mesh_headings: Neisseria meningitidis, Serogroup B--genetics mesh_headings: Neisseria meningitidis, Serogroup B--isolation & purification mesh_headings: Phylogeny mesh_headings: Time Factors citation: de Filippis, I and de Lemos, APS and Hostetler, JB and Wollenberg, K and Sacchi, CT and Harrison, LH and Bash, MC and Prevots, DR (2012) Molecular epidemiology of neisseria meningitidis serogroup B in Brazil. PLoS ONE, 7 (3). document_url: http://d-scholarship-dev.library.pitt.edu/14156/1/Molecular_Epidemiology.pdf document_url: http://d-scholarship-dev.library.pitt.edu/14156/8/licence.txt