TY  - JOUR
ID  - pittir14003
UR  - http://d-scholarship-dev.library.pitt.edu/14003/
IS  - 12
A1  - Choi, YJ
A1  - Ghedin, E
A1  - Berriman, M
A1  - McQuillan, J
A1  - Holroyd, N
A1  - Mayhew, GF
A1  - Christensen, BM
A1  - Michalski, ML
Y1  - 2011/12/01/
N2  - Background: Developing intervention strategies for the control of parasitic nematodes continues to be a significant challenge. Genomic and post-genomic approaches play an increasingly important role for providing fundamental molecular information about these parasites, thus enhancing basic as well as translational research. Here we report a comprehensive genome-wide survey of the developmental transcriptome of the human filarial parasite Brugia malayi. Methodology/Principal Findings: Using deep sequencing, we profiled the transcriptome of eggs and embryos, immature (?3 days of age) and mature microfilariae (MF), third- and fourth-stage larvae (L3 and L4), and adult male and female worms. Comparative analysis across these stages provided a detailed overview of the molecular repertoires that define and differentiate distinct lifecycle stages of the parasite. Genome-wide assessment of the overall transcriptional variability indicated that the cuticle collagen family and those implicated in molting exhibit noticeably dynamic stage-dependent patterns. Of particular interest was the identification of genes displaying sex-biased or germline-enriched profiles due to their potential involvement in reproductive processes. The study also revealed discrete transcriptional changes during larval development, namely those accompanying the maturation of MF and the L3 to L4 transition that are vital in establishing successful infection in mosquito vectors and vertebrate hosts, respectively. Conclusions/Significance: Characterization of the transcriptional program of the parasite's lifecycle is an important step toward understanding the developmental processes required for the infectious cycle. We find that the transcriptional program has a number of stage-specific pathways activated during worm development. In addition to advancing our understanding of transcriptome dynamics, these data will aid in the study of genome structure and organization by facilitating the identification of novel transcribed elements and splice variants. © 2011 Choi et al.
JF  - PLoS Neglected Tropical Diseases
VL  - 5
SN  - 1935-2727
TI  - A deep sequencing approach to comparatively analyze the transcriptome of lifecycle stages of the filarial worm, brugia malayi
AV  - public
ER  -