relation: http://d-scholarship-dev.library.pitt.edu/13277/
title: HIV-1 infection of DC: Evidence for the acquisition of virus particles from infected T cells by antigen uptake mechanism
creator: Venkatachari, NJ
creator: Alber, S
creator: Watkins, SC
creator: Ayyavoo, V
description: Dendritic cells (DC) play a pivotal role in transmission and dissemination of HIV-1. Earlier studies reported that DC present at the site of infection trap virus particles via DC-SIGN and transfer the virus to the interacting naïve T cells. This prompted us to ask the question whether DC could acquire virus from infected T cells during DC-T cell interaction. To address this, we investigated the likely transfer of virus from HIV-1 infected T cells to DC and the underlying mechanisms involved. Results indicate that DC acquire virus from infected T cells via antigen uptake mechanism and this results in infection of DC with expression of proteins directed by viral DNA. Further studies with HIV-1 lacking the Env protein also resulted in infection of DC. The use of antibodies against DC-SIGN and DC-SIGN-R ruled out a role for receptor in the infection of DC. Additional data show that DC infection is directly correlated with the ability of DC to take up antigen from infected T cells. Overall, these studies provide evidence to suggest that HIV-1, besides infecting immune cells, also utilizes immunological mechanism(s) to acquire and disseminate virus. © 2009 Venkatachari et al.
date: 2009-10-15
type: Article
type: PeerReviewed
format: application/pdf
language: en
rights: attached
identifier: http://d-scholarship-dev.library.pitt.edu/13277/1/HIV-1_Infection.pdf
format: text/plain
language: en
rights: attached
identifier: http://d-scholarship-dev.library.pitt.edu/13277/8/licence.txt
identifier:   Venkatachari, NJ and Alber, S and Watkins, SC and Ayyavoo, V  (2009) HIV-1 infection of DC: Evidence for the acquisition of virus particles from infected T cells by antigen uptake mechanism.  PLoS ONE, 4 (10).       
relation: 10.1371/journal.pone.0007470