relation: http://d-scholarship-dev.library.pitt.edu/11902/
title: Capillary HPLC Separation of Selected Neuropeptides
creator: Yi, Ying
description: Neuropeptides play a pivotal role in brain and peripheral nervous system function. As high performance liquid chromatography (HPLC) becomes the central tool in the separation and characterization of peptide and protein samples, its selectivity optimization has attracted increasing attention. This research program aims to develop useful, quantitative analysis methods for neuropeptides and their hydrolysis fragments by capillary HPLC. Related peptide pairs are successfully separated, such as leu-enkephalin and [Des-Tyr1] leu-enkephalin, dynorphin A and dynorphin B, galanin and its fragment Gal1-16. The hydrolysis of leu-enkephalin to [Des-Tyr1] leu-enkephalin by organotypic hippocampal slice cultures (OHSCs) can be monitored by the same HPLC system. The separation of seven hippocampal neuropeptides with similar hydrophobicity, Bj-PRO-5a, [Des-Tyr1] leu-enkephalin, leu-enkephalin, pentagastrin, Antho-RW-amide I, dynorphin A 1-6 and angiotensin II, is accomplished by thermally tuned tandem capillary columns (T3C). The chromatographic selectivity is continuously, systematically and significantly optimized by individual adjustment of each column’s temperature. The T3C concept is applied for the first time with capillary columns, which is an important step towards optimization of selectivity for separations of small samples by liquid chromatography.
date: 2012-06-08
type: University of Pittsburgh ETD
type: PeerReviewed
format: application/pdf
language: en
identifier: http://d-scholarship-dev.library.pitt.edu/11902/4/YiY_etdPitt_2012.pdf
identifier:   Yi, Ying  (2012) Capillary HPLC Separation of Selected Neuropeptides.  Master's Thesis, University of Pittsburgh.    (Unpublished)