Boyden, MN and Asher, SA
(2001)
UV Raman studies of peptide conformation demonstrate that betanova does not cooperatively unfold.
Biochemistry, 40 (45).
13723 - 13727.
ISSN 0006-2960
![[img]](http://d-scholarship-dev.library.pitt.edu/style/images/fileicons/text_plain.png) |
Plain Text (licence)
Available under License : See the attached license file.
Download (1kB)
|
Abstract
We used UV resonance Raman spectroscopy (UVRR) excited within the peptide bond π→π*electronic transitions and within the aromatic amino acid π→π*electronic transitions to examine the temperature dependence of the solution conformation of betanova, a 20-residue β-sheet polypeptide [Kortemme, T., Ramirez-Alvarado, M., and Serrano, L. (1998) Science 281, 253-256]. The 206.5 nm excited UVRR enhances the amide vibrations and demonstrates that betanova has a predominantly β-sheet structure between 5 and 82 °C. The 229 nm excited UVRR, which probes the tyrosine and tryptophan side chain vibrations, shows an increase in the solvent exposure of the tryptophan side chains as the temperature is increased. Our results are consistent with the existence of an intermediate state similar to that calculated by Bursulaya and Brooks [Bursulaya, B. D., and Brooks, C. L. (1999) J. Am. Chem. Soc. 121, 9947-9951] and exclude the previously proposed two-state cooperative folding mechanism. Betanova's structure appears to be molten globule over the 3-82 °C temperature range of our study.
Share
Citation/Export: |
|
Social Networking: |
|
Details
Item Type: |
Article
|
Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID ![](/images/orcid_id_24x24.png) |
---|
Boyden, MN | | | | Asher, SA | asher@pitt.edu | ASHER | |
|
Date: |
13 November 2001 |
Date Type: |
Publication |
Journal or Publication Title: |
Biochemistry |
Volume: |
40 |
Number: |
45 |
Page Range: |
13723 - 13727 |
DOI or Unique Handle: |
10.1021/bi011505k |
Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
Refereed: |
Yes |
ISSN: |
0006-2960 |
MeSH Headings: |
Circular Dichroism; Peptides--chemistry; Protein Folding; Protein Structure, Secondary; Proteins--chemistry; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman--methods |
PubMed ID: |
11695921 |
Date Deposited: |
20 Mar 2013 16:43 |
Last Modified: |
02 Feb 2019 16:55 |
URI: |
http://d-scholarship-dev.library.pitt.edu/id/eprint/17789 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Altmetric.com
Actions (login required)
![View Item View Item](/style/images/action_view.png) |
View Item |