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UV resonance raman measurements of poly-L-lysine's conformational energy landscapes: Dependence on perchlorate concentration and temperature

Lu, M and Ahmed, Z and Mikhonin, AV and Asher, SA (2007) UV resonance raman measurements of poly-L-lysine's conformational energy landscapes: Dependence on perchlorate concentration and temperature. Journal of Physical Chemistry B, 111 (26). 7675 - 7680. ISSN 1520-6106

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Abstract

UV resonance Raman spectroscopy has been used to determine the conformational energy landscape of polyL-lysine (PLL) in the presence of NaClO4 as a function of temperature. At 1 °C, in the presence of 0.83 M NaClO4, PLL shows an ∼86% α-helix-like content, which contains ́-helix and π-bulge/helix conformations. The high a-helix-like content of PLL occurs because of charge screening due to strong ion-pair formation between ClO4- and the lysine side chain -NH3+. As the temperature increases from 1 to 60 °C, the ́-helix and π-bulge/helix conformations melt into extended conformations (PPII and 2.51-helix). We calculate the Ψ Ramachandran angle distribution of the PLL peptide bonds from the UV Raman spectra which allows us to calculate the PLL (un)folding energy landscapes along the Ψ reaction coordinate. We observe a basin in the Ψ angle conformational space associated with α-helix and π-bulge/helix conformations and another basin for the extended PPII and 2.5 1-helical conformations. © 2007 American Chemical Society.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lu, M
Ahmed, Z
Mikhonin, AV
Asher, SAasher@pitt.eduASHER
Date: 5 July 2007
Date Type: Publication
Journal or Publication Title: Journal of Physical Chemistry B
Volume: 111
Number: 26
Page Range: 7675 - 7680
DOI or Unique Handle: 10.1021/jp0703758
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 1520-6106
MeSH Headings: Circular Dichroism; Perchloric Acid--chemistry; Polylysine--chemistry; Protein Conformation; Protein Folding; Sodium Compounds--chemistry; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman; Temperature; Thermodynamics
PubMed ID: 17567063
Date Deposited: 08 Feb 2013 21:06
Last Modified: 12 Jun 2021 23:55
URI: http://d-scholarship-dev.library.pitt.edu/id/eprint/17221

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