Mikhonin, AV and Myshakina, NS and Bykov, SV and Asher, SA
(2005)
UV resonance Raman determination of polyproline II, extended 2.5 <inf>1</inf>-helix, and β-sheet ψ angle energy landscape in poly-L-lysine and poly-L-glutamic acid.
Journal of the American Chemical Society, 127 (21).
7712 - 7720.
ISSN 0002-7863
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Abstract
UV resonance Raman (UVR) spectroscopy was used to examine the solution conformation of poly-L-lysine (PLL) and poly-L-glutamic acid (PGA) in their non-α-helical states. UVR measurements indicate that PLL (at pH = 2) and PGA (at pH = 9) exist mainly in a mixture of polyproline II (PPII) and a novel left-handed 2.51-helical conformation, which is an extended β-strand-like conformation with Ψ ≈ 1170° and Φ ≈ 130°. Both of these conformations are highly exposed to water. The energies of these conformations are very similar. We see no evidence of any disordered "random coil" states. In addition, we find that a PLL and PGA mixture at neutral pH is ∼60% β-sheet and contains PPII and extended 2.5 1-helix conformations. The β-sheet conformation shows little evidence of amide backbone hydrogen bonding to water. We also developed a method to estimate the distribution of Ψ Ramachandran angles for these conformations, which we used to estimate a Ψ Ramachandran angle energy landscape. We believe that these are the first experimental studies to give direct information on protein and peptide energy landscapes. © 2005 American Chemical Society.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID ![](/images/orcid_id_24x24.png) |
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Mikhonin, AV | | | | Myshakina, NS | | | | Bykov, SV | | | | Asher, SA | asher@pitt.edu | ASHER | |
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Date: |
1 June 2005 |
Date Type: |
Publication |
Journal or Publication Title: |
Journal of the American Chemical Society |
Volume: |
127 |
Number: |
21 |
Page Range: |
7712 - 7720 |
DOI or Unique Handle: |
10.1021/ja044636s |
Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
Refereed: |
Yes |
ISSN: |
0002-7863 |
MeSH Headings: |
Circular Dichroism; Models, Molecular; Peptides--chemistry; Polyglutamic Acid--chemistry; Polylysine--chemistry; Protein Conformation; Protein Folding; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman; Thermodynamics |
PubMed ID: |
15913361 |
Date Deposited: |
08 Feb 2013 21:14 |
Last Modified: |
12 Jun 2021 23:55 |
URI: |
http://d-scholarship-dev.library.pitt.edu/id/eprint/17209 |
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