Asher, SA and Mikhonin, AV and Bykov, S
(2004)
UV Raman demonstrates that α-helical polyalanine peptides melt to polyproline II conformations.
Journal of the American Chemical Society, 126 (27).
8433 - 8440.
ISSN 0002-7863
|
Plain Text (licence)
Available under License : See the attached license file.
Download (1kB)
|
Abstract
We examined the 204-nm UV Raman spectra of the peptide XAO, which was previously found by Shi et al.'s NMR study to occur in aqueous solution in a polyproline II (PPII) conformation (Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 9190). The UV Raman spectra of XAO are essentially identical to the spectra of small peptides such as ala5 and to the large 21 -residue predominantly Ala peptide, AP. We conclude that the non-α-helical conformations of these peptides are dominantly PPII. Thus, AP, which is highly α-helical at room temperature, melts to a PPII conformation. There is no indication of any population of intermediate disordered conformations. We continued our development of methods to relate the Ramachandran ψ-angle to the amide III band frequency. We describe a new method to estimate the Ramachandran ψ-angular distributions from amide III band line shapes measured in 204-nm UV Raman spectra. We used this method to compare the ψ-distributions in XAO, ala5, the non-α-helical state of AP, and acid-denatured apomyoglobin. In addition, we estimated the ψ-angle distributions of peptide bonds which occur in non-α-helix and non-β-sheet conformations in a small library of proteins.
Share
Citation/Export: |
|
Social Networking: |
|
Details
Item Type: |
Article
|
Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID |
---|
Asher, SA | asher@pitt.edu | ASHER | | Mikhonin, AV | | | | Bykov, S | | | |
|
Date: |
14 July 2004 |
Date Type: |
Publication |
Journal or Publication Title: |
Journal of the American Chemical Society |
Volume: |
126 |
Number: |
27 |
Page Range: |
8433 - 8440 |
DOI or Unique Handle: |
10.1021/ja049518j |
Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
Refereed: |
Yes |
ISSN: |
0002-7863 |
MeSH Headings: |
Amides--chemistry; Chemistry, Physical; Peptides--chemistry; Physicochemical Phenomena; Protein Conformation; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman--methods; Temperature |
PubMed ID: |
15238000 |
Date Deposited: |
08 Feb 2013 21:21 |
Last Modified: |
12 Jun 2021 23:55 |
URI: |
http://d-scholarship-dev.library.pitt.edu/id/eprint/17176 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Altmetric.com
Actions (login required)
|
View Item |